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Plant Cell, Tissue and Organ Culture

, Volume 51, Issue 1, pp 17–25 | Cite as

In vitro plant regeneration from embryogenic cultures of a diploid and a triploid, Cavendish banana

  • Cuauhtémoc Navarro
  • Rosa Ma. Escobedo
  • Alberto Mayo
Article

Abstract

Plant regeneration by somatic embryogenesis was attempted with diploid (Musa acuminata ssp. malaccensis) and triploid ('Grand Nain') bananas. Explants inoculated in vitro were, respectively, immature zygotic embryos and male flower bud primordia. An histological study showed that the embryogenic process involves a sequence of similar events for both species. A yellow-green compact callus was initiated, which consisted of an actively dividing meristematic zone surrounded by several layers of starchy cells. A white and friable callus, characterized by the presence of proembryonic cells, bicellular proembryos and proembryonal masses in its periphery gradually appeared, which finally gave rise to somatic embryos from which plants were recovered. Induction media contained 2,4-D (and also NAA and IAA for the triploid); zeatin and kinetin were necessary for embryo maturation and 6-BA and IAA were used for germination.

histology Musa acuminata somatic embryogenesis regeneration 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Cuauhtémoc Navarro
    • 1
  • Rosa Ma. Escobedo
    • 1
  • Alberto Mayo
    • 1
  1. 1.Departamento de Biotecnología, Centro de Investigación Científica de Yucatán, A.C., Apartado 87 CORDEMEXMérida, YucatánMéxico

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