Plant Molecular Biology

, Volume 36, Issue 4, pp 601–612

Expression of fission yeast cdc25 alters the frequency of lateral root formation in transgenic tobacco

  • Rowan S. McKibbin
  • Nigel G. Halford
  • Dennis Francis
Article

DOI: 10.1023/A:1005937011095

Cite this article as:
McKibbin, R.S., Halford, N.G. & Francis, D. Plant Mol Biol (1998) 36: 601. doi:10.1023/A:1005937011095

Abstract

Lateral root formation was examined following the expression of a fission yeast mitotic regulator gene, cdc25, under the control of a tetracycline-inducible promoter in cultured roots of tobacco. Over expression of cdc25 in fission yeast results in premature cell division at a reduced cell size. Our aim was to examine whether cdc25 expression would affect cell size in the tobacco roots, and what effect this would have on lateral root morphogenesis. Transgene integration was confirmed by Southern blotting; it was inherited as a dominant Mendelian trait. Conditions for optimal expression, determined using plants transformed with gus under the control of the same promoter, were: addition of tetracycline (5 µg/ml) every 72 h, to cultured roots in Murashige-Skoog liquid medium in darkness at 27 °C. After the addition of tetracycline, cdc25 transcripts were detected using RT-PCR, initially after 48 h, and more strongly after 72 h. Appearance of cdc25 transcripts was followed by major changes in the roots. Compared with controls, lateral root primordia were initiated more frequently, were significantly smaller and comprised smaller cells at mitosis. However, cdc25 expression did not perturb normal development of the lateral roots. The data are consistent with cdc25 expression leading to a greater frequency of lateral root primordium formation and establishing a new threshold size for cell division in the primordia which was then maintained throughout subsequent development.

cell division cell size lateral roots 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Rowan S. McKibbin
    • 1
  • Nigel G. Halford
    • 1
  • Dennis Francis
    • 2
  1. 1.IACR – Long Ashton Research Station, Department of Agricultural SciencesUniversity of Bristol, Long AshtonBristolUK
  2. 2.School of Pure and Applied BiologyUniversity of WalesCardiffUK

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