Plant Molecular Biology

, Volume 36, Issue 2, pp 323–328

Analysis of the isopentenyl diphosphate isomerase gene family from Arabidopsis thaliana

  • Michael Campbell
  • Frederick M. Hahn
  • C. Dale Poulter
  • Thomas Leustek
Article

DOI: 10.1023/A:1005935516274

Cite this article as:
Campbell, M., Hahn, F.M., Poulter, C.D. et al. Plant Mol Biol (1998) 36: 323. doi:10.1023/A:1005935516274

Abstract

Two Arabidopsis thaliana cDNAs (IPP1 and IPP2) encoding isopentenyl diphosphate isomerase (IPP isomerase) were isolated by complementation of an IPP isomerase mutant strain of Saccharomyces cerevisiae. Both cDNAs encode enzymes with an amino terminus that may function as a transit peptide for localization in plastids. At least 31 amino acids from the amino terminus of the IPP1 protein and 56 amino acids from the amino terminus of the IPP2 protein are not essential for enzymatic activity. Genomic DNA blot analysis confirmed that IPP1 and IPP2 are derived from a small gene family in A. thaliana. Based on northern analysis expression of both cDNAs occurs predominantly in roots of mature A. thaliana plants grown to the pre-flowering stage.

isoprenoid biosynthetic pathway functional complementation isopentenyl diphosphate isomerase higher plants yeast 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Michael Campbell
    • 1
  • Frederick M. Hahn
    • 2
  • C. Dale Poulter
    • 2
  • Thomas Leustek
    • 3
  1. 1.Division of SciencePennsylvania State University-Erie, The Behrend CollegeErieUSA
  2. 2.Department of ChemistryUniversity of Utah, SaltLake CityUSA
  3. 3.Center for Agricultural Molecular BiologyRutgers UniversityNew BrunswickUSA

Personalised recommendations