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Plant Cell, Tissue and Organ Culture

, Volume 51, Issue 2, pp 127–135 | Cite as

Effects of genotype, explant source and growth regulators on organogenesis in carnation callus

  • Henni Kallak
  • Maere Reidla
  • Ille Hilpus
  • Kai Virumäe
Article

Abstract

For callus induction, shoot tips and nodal or internodal stem segments of carnation cultivars (Coral, Jaguar, Salome and Sarinah) were grown on MS basal medium with 2,4-dichlorophenoxyacetic acid and kinetin. To achieve organogenesis, calli were transferred onto MS medium without or with growth regulators (indoleacetic acid, naphthaleneacetic acid, indolebutyric acid, kinetin, benzyladenine) in different combinations. Shoot primordia emerged from the subsurface meristemoids of calli, roots developed from the inner callus cells. The effects of genotype, explant source and growth regulators on callus-mediated organogenesis differently manifested themselves in caulogenesis and rhizogenesis, respectively. The number of root-forming calli most of all depended on genotype and least of all on explant source. Unlike rhizogenesis, caulogenesis essentially depended on explant source: internodal calli of all the tested cultivars practically missed the shoot formation ability. The number of caulogenetic calli from apical-nodal segments significantly depended on genotype, but was also affected by growth regulators.

callogenesis caulogenesis Dianthus caryophyllus genetic factors rhizogenesis 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Henni Kallak
    • 1
  • Maere Reidla
    • 1
  • Ille Hilpus
    • 1
  • Kai Virumäe
    • 1
  1. 1.Institute of Molecular and Cell BiologyUniversity of TartuTartuEstonia

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