Plant Cell, Tissue and Organ Culture

, Volume 51, Issue 3, pp 229–232 | Cite as

Plant regeneration from peduncle segments of oil seed Brassica species: Influence of silver nitrate and silver thiosulfate

  • Susan Eapen
  • Leela George


Cultured peduncle segments of B. juncea, B. campestris, B. napus, B. nigra and B. carinata produced shoot buds on Murashige and Skoog medium supplemented with benzyladenine and 1-naphthalene acetic acid. Supplementation of the media with 30 µm silver nitrate or silver thiosulfate enhanced the frequency of shoot regeneration. The regenerated shoots could be rooted at a frequency of 95% and transferred to soil where 75% survived and set seed.

Brassica juncea Brassica campestris Brassica nigra Brassica carinata 


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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Susan Eapen
    • 1
  • Leela George
    • 1
  1. 1.Biotechnology Divisin, Bhabha Atomic Research Centre, TrombayBombayIndia

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