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Plant Cell, Tissue and Organ Culture

, Volume 51, Issue 3, pp 229–232 | Cite as

Plant regeneration from peduncle segments of oil seed Brassica species: Influence of silver nitrate and silver thiosulfate

  • Susan Eapen
  • Leela George
Article

Abstract

Cultured peduncle segments of B. juncea, B. campestris, B. napus, B. nigra and B. carinata produced shoot buds on Murashige and Skoog medium supplemented with benzyladenine and 1-naphthalene acetic acid. Supplementation of the media with 30 µm silver nitrate or silver thiosulfate enhanced the frequency of shoot regeneration. The regenerated shoots could be rooted at a frequency of 95% and transferred to soil where 75% survived and set seed.

Brassica juncea Brassica campestris Brassica nigra Brassica carinata 

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References

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Susan Eapen
    • 1
  • Leela George
    • 1
  1. 1.Biotechnology Divisin, Bhabha Atomic Research Centre, TrombayBombayIndia

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