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Plant Molecular Biology

, Volume 37, Issue 3, pp 577–580 | Cite as

Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. PCC 6803

  • Ghada Ajlani
  • Claudie Vernotte
Article

Abstract

A mutant strain of the cyanobacterium Synechocystis PCC 6803, called PAL, (PC-, ΔapcAB, ΔapcE), lacking phycocyanin, allophycocyanin and the core-membrane linker (Lcm), was constructed. The strain was characterized by absorption and fluorescence spectroscopy. The mutant compensates for the absence of the major PS II antenna by increasing its PS II / PS I ratio. It is stable and grows well albeit more slowly than wild type.

apc genes fluorescence gene inactivation photosystems phycobiliproteins Synechocystis 

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References

  1. 1.
    Ajlani G, Kirilovsky D, Picaud M, Astier C: Molecular analysis of psbA mutations responsible for various herbicide resistance phenotype in Synechocystis 6714. Plant Mol Biol 13: 469–479 (1989).PubMedGoogle Scholar
  2. 2.
    Ajlani G, Vernotte C, DiMagno L, Haselkorn R: Phycobilisome core mutants of Synechocystis PCC 6803. Biochim Biophys Acta 1231: 189–196 (1995).Google Scholar
  3. 3.
    Bruce D, Brimble S, Bryant DA: State transitions in a phycobilisomelessmutant of the cyanobacteriumSynechococcus sp. PCC 7002. Biochim Biophys Acta 974: 66–73 (1989).PubMedGoogle Scholar
  4. 4.
    Bryant DA: Cyanobacterial phycobilisomes: progress towards a complete structural and functional analysis via molecular genetics In: Bogorad L, Vasil IK (eds) The Molecular Biology of Plastids and Mitochondria, vol. 7B, pp. 257–300, Academic Press, New York (1991).Google Scholar
  5. 5.
    Capuano V, Braux A, Tandeau de Marsac N, Houmard J: The ‘anchor polypeptide’ of cyanobacterial phycobilisomes. Molecular characterization of the Synechococcus sp. PCC6301 apcE gene. J Biol Chem 266: 7239–7247 (1991).PubMedGoogle Scholar
  6. 6.
    Capuano V, Thomas JC, Tandeau de Marsac N, Houmard J: An in vivo approach to define the role of the LCM, the key polypeptide of cyanobacterial phycobilisome. J Biol Chem 268: 8277–8283 (1993).PubMedGoogle Scholar
  7. 7.
    DiMagno L, Haselkorn R: Isolation and characterisation of the genes encoding allophycocyanin subunits and two linker proteins from Synechocystis 6714. Plant Mol Biol 21: 835–845 (1993).PubMedGoogle Scholar
  8. 8.
    Elhai J, Wolk CP: Aversatile class of positiveselection vectors based on the nonviability of palindromecontaining plasmids that allows cloning into long polylinkers. Gene 68: 119–138 (1988).CrossRefPubMedGoogle Scholar
  9. 9.
    Elmorjani K, Thomas JC, Sebban P: Phycobilisomes of wild type and pigmentmutants of the cyanobacterium Synechocystis PCC 6803. Arch Microbiol 146: 186–191 (1986).Google Scholar
  10. 10.
    Herdman M, Delaney SF, Carr NG: A new medium for the isolation and growth of auxotrophic mutants of the blue green alga Anacystis nidulans. J Gen Microbiol 79: 233–237 (1973).Google Scholar
  11. 11.
    Plank T, Toole C, Anderson L: Subunit interactions and protein stability in the cyanobacterial lightharvesting proteins. J Bact 177: 6798–6803 (1995).PubMedGoogle Scholar
  12. 12.
    Rögner M, Nixon P, Diner B: Purification and characterization of Photosystem I and Photosystem II core complexes from wild-type and phycocyanindeficient strains of the cyanobacterium Synechocystis PCC 6803 J Biol Chem 265: 6189–6196 (1990).PubMedGoogle Scholar
  13. 13.
    Shen G, Boussiba S, Vermaas FJ: Synechocystis sp. PCC 6803 strains lacking photosystem I and phycobilisome function. Plant Cell 5: 1853–1863 (1993).PubMedGoogle Scholar
  14. 14.
    Sidler WA: Phycobilisome and Phycobiliprotein structures In: Bryant DA (eds) The Molecular Biology of Cyanobacteria pp. 139–216, Kluwer Academic Publishers, Dordrecht, Netherlands (1994).Google Scholar
  15. 15.
    Su X, Goodman Fraenkel P, Bogorad L: Excitation energy tranfer from phycocyanin to chlorophyll in an apcAdefective mutant of Synechocystis sp. PCC 6803. J Biol Chem 267: 22944–22950 (1992).PubMedGoogle Scholar
  16. 16.
    Vernotte C, Picaud M, Kirilovsky D, Olive J, Ajlani G, Astier C: Changes in the photosynthetic apparatus in the cyanobacterium Synechocystis sp. PCC 6714 following lighttodark and darktolight transitions. Photosynth Res 32: 45–57 (1992).Google Scholar
  17. 17.
    Williams JG: Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803. Meth Enzymol 167: 766–778 (1988).Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Ghada Ajlani
    • 2
  • Claudie Vernotte
    • 1
  1. 1.Centre de Génétique Moléculaire du Centre National de la Recherche Scientifique, associé à l'Université Pierre et Marie Curie, Photosynthèse Bactérienne, Bât. 24Gif-sur-Yvette, CedexFrance
  2. 2.Grenoble Cedex 09France

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