Systematic Parasitology

, Volume 40, Issue 2, pp 81–92 | Cite as

Allozyme and morphological identification of shape Anisakis, Contracaecum and Pseudoterranova from Japanese waters (Nematoda, Ascaridoidea)

  • Simonetta Mattiucci
  • Lia Paggi
  • Giuseppe Nascetti
  • Hajime Ishikura
  • Kokichi Kikuchi
  • Noriyuki Sato
  • Rossella Cianchi
  • Luciano Bullini


Allozyme markers were used to identify anisakid nematodes from marine Japanese waters, morphologically assigned to three species complexes: Anisakis simplex (s. l.), Contracaecum osculatum (s. l.) and Pseudoterranova decipiens (s. l.). Samples assigned to A. simplex (s. l.) were found to correspond genetically to A. simplex sensu stricto, those of C. osculatum (s. l.) to C. osculatum A. No morphological characters are yet available to distinguish sibling species of these two complexes. As to the P. decipiens complex, two distinct species were detected: the first corresponded to P. decipiens C, previously recovered in the northern Atlantic, the second to P. decipiens D from Japan. The two species are genetically well differentiated, with five of the 19 loci tested showing distinct fixed alleles. Their reproductive isolation was proved by the lack of hybrids or recombinants in sympatric samples recovered from the same definitive host, Erignathus barbatus. P. decipiens D was found to correspond morphologically to Porrocaecum azarasi, previously considered a synonym of P. decipiens. Accordingly, the name Pseudoterranova azarasi (Yamaguti & Arima, 1942) n. comb. is proposed for P. decipiens D. Similarly, P. decipiens C fits in general morphology, type-locality and host with Ascaris bulbosa, also previously considered a synonym of P. decipiens. The name Pseudoterranova bulbosa (Cobb, 1888) n. comb. is proposed for P. decipiens C.


Morphological Character Species Complex Reproductive Isolation Distinct Species Ascaris 
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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Simonetta Mattiucci
    • 1
  • Lia Paggi
    • 1
  • Giuseppe Nascetti
    • 2
  • Hajime Ishikura
    • 3
  • Kokichi Kikuchi
    • 3
  • Noriyuki Sato
    • 3
  • Rossella Cianchi
    • 4
  • Luciano Bullini
    • 4
  1. 1.Institute of ParasitologyUniversity of Rome “La Sapienza”RomeItaly
  2. 2.Department of Environmental SciencesTuscia UniversityViterboItaly
  3. 3.Department of Pathology, Sapporo Medical CollegeUniversity School of MedicineSapporoJapan
  4. 4.Department of Genetics and Molecular BiologyUniversity of Rome “La Sapienza”RomeItaly

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