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Plant Molecular Biology

, Volume 37, Issue 3, pp 505–511 | Cite as

Molecular cloning and characterization of starch-branching enzyme II from potato

  • Clas-Tomas Larsson
  • Jamshid Khoshnoodi
  • Bo Ek
  • Lars Rask
  • Håkan Larsson
Article

Abstract

Full-length cDNA for starch branching enzyme (SBE) II of potato was isolated and sequenced. In potato, similary to most other investigated plants, the SBE-II isoform differs from SBE-I by having an acidic amino-terminal extension and a shorter carboxyterminus. Two forms of SBE-II, migrating as 98 and 95 kDa proteins in 6% SDS-polyacrylamide gels, were associated to tuber starch. The latter form was 16 amino acids shorter in the amino terminus. Transcript of SBE-II was present in leaf tissue, whereas significant expression was not seen in tubers. On the other hand, a significant amount of SBE-I transcript was detected in tuber tissue but not in leaves.

branching enzyme expression potato Solanum tuberosum starch 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Clas-Tomas Larsson
    • 1
  • Jamshid Khoshnoodi
    • 1
  • Bo Ek
    • 1
  • Lars Rask
    • 2
  • Håkan Larsson
    • 1
  1. 1.Uppsala Genetic Center, Dept. of Cell ResearchSwedish University of Agricultural SciencesUppsalaSweden
  2. 2.Dept. of Medical and Physiological ChemistryUppsala UniversityUppsalaSweden

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