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Plant Cell, Tissue and Organ Culture

, Volume 49, Issue 1, pp 75–79 | Cite as

Effect of picloram and methyl jasmonate on growth and taxane accumulation in callus culture of Taxus × media var. Hatfieldii

  • Miroslawa Furmanowa
  • Kazimierz Glowniak
  • Katarzyna Syklowska-Baranek
  • Grazyna Zgórka
  • Aleksandra Józefczyk
Article

Abstract

We have analysed the effect of some culture conditions and media components on callus growth rate and production of taxanes in callus of Taxus × media var. Hatfieldii. For callus induction and maintenance a Gamborg B5 medium and a White - Rangaswamy medium (WR) with different modifications were used. On an improved WR medium (containing 10 μM picloram) the callus growth factor increased up to 5.8 fold (fresh weight). Picloram only enhanced the growth of callus, but not taxane production. On WR medium with (100 μM) methyl jasmonate the paclitaxel content increased from 2.37 μg g-1 to 90 μg g-1 and cephalomannine from 5.14 μg g-1 to 29.14 μg g-1 (dry weight), whereas growth of the cultures ceased. The presence of paclitaxel and cephalomannine was established by high performance liquid chromatography.

methyl jasmonate paclitaxel picloram tissue culture 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Miroslawa Furmanowa
    • 1
  • Kazimierz Glowniak
    • 2
  • Katarzyna Syklowska-Baranek
    • 1
  • Grazyna Zgórka
    • 2
  • Aleksandra Józefczyk
    • 2
  1. 1.Dept.of Biology and PharmBot. Medical AcademyWarsawPoland
  2. 2.Dept. of PharmacognosyMedical AcademyPoland

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