Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 48, Issue 1, pp 45–52 | Cite as

Effects of thidiazuron and benzyladenine on axillary shoot proliferation of three green ash (Fraxinus pennsylvanica Marsh.) clones

  • Mee-Sook Kim
  • Carol M. Schumann
  • Ned B. Klopfenstein
Article

Abstract

Mature seeds of three green ash (Fraxinus pennsylvanica Marsh.) clones, SD1009 (South Dakota origin), SD2002 (South Dakota origin), and KA2018 (Kansas origin) were cut to remove the apical portion and germinated on Murashige and Skoog (1962) salts with B5 vitamins (Gamborg et al., 1968) (MSB5) medium without plant growth regulators. Stable axillary shoot establishment was achieved for all three clones by subculture on MSB5 medium containing a combination of 5 μM thidiazuron (TDZ), 5 μM 6-benzyladenine (BA), and 1 μM indole-3-butyric acid (IBA). Following shoot establishment, axillary shoots were placed on MSB5 medium containing a single treatment of TDZ (1, 5, 10, 20, or 40 μM) or BA (1, 5, 10, 20, 40, or 80 μM). Concentration of TDZ and BA significantly affected shoot biomass (total dry weight of axillary shoots), with 10 μm TDZ or 40 μm BA providing maximum shoot proliferation with all three clones. Significant clonal differences also were noted in the proliferation of axillary shoots, with clone SD1009 exhibiting the highest axillary shoot proliferation. Axillary shoots were rooted under ex vitro conditions and acclimatized to the greenhouse.

in vitro culture micropropagation woody plant tissue culture 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arrillaga IV, Lerman V & Segura J (1992) Micropropagation of juvenile and adult flowering ash. Amer. Soc. Hort. Sci. 117: 346–350Google Scholar
  2. Bates S, Preece JE, Navarrete NE, Van Sambeek JW & Gaffeny GR (1992) Thidiazuron stimulates shoot organogenesis and somatic embryogenesis in white ash (Fraxinus americana L.). Plant Cell Tiss. Org. Cult. 31: 21–29Google Scholar
  3. Chalupa V (1988) Large scale micropropagation of Quercus robur L. using adenine-type cytokinins and thidiazuron to stimulate shoot proliferation. Biol. Plant (Praha) 30: 414–421Google Scholar
  4. Driver JA & Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. HortSci. 19: 507–509Google Scholar
  5. Fellman CD, Read PE & Hosier MA (1987) Effect of thidiazuron and CPPU on meristem formation and shoot proliferation. HortSci. 22: 1197–1200Google Scholar
  6. Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean roots cells. Exp. Cell Res. 50: 151–158Google Scholar
  7. Hammatt N (1994) Shoot initiation in the leaflet axils of compound leaves from micropropagated shoots of juvenile and mature common ash (Fraxinus excelsior L.). J. Experi. Bot. 45 (275): 871–875Google Scholar
  8. Hammatt N & Ridout MS (1992) Micropropagation of common ash (Fraxinus excelsior). Plant Cell Tiss. Org. Cult. 13: 67–74Google Scholar
  9. Heller R (1953) Recherches sur la nutrition minerale des tissue vegetaux cultives in vitro. Ann. Sci. Nat. Bot. Biol. Veg. 14: 1–223Google Scholar
  10. Huetteman CA & Preece JE (1993) Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33: 105–119Google Scholar
  11. Kim M-S, Schumann CM, Cregg BM & Klopfenstein NB (1994) Micropropagation and regeneration systems of green ash (Fraxinus pennsylvanica Marsh). In: Michler CH, Becwar MR, Cullen D, Nance WL, Sederoff RR & Slavicek JM (eds) Applications of Biotechnology to Tree Culture, Protection, and Utilization. Abstract (p 74). USDA Forest Service, North Central Forest Experiment Station, Gen. Tech. Rep. NC-175Google Scholar
  12. Kim M-S (1995) Micropropagation of green ash (Fraxinus pennsylvanica Marsh.): A comparative study of three genotypes. M.S. thesis, University of Nebraska, Lincoln, NE, U.S.A. (98p)Google Scholar
  13. Libby WJ & Ahuja MR (1993) Micropropagation and clonal options in forestry. In: Ahuja MR (ed) Micropropagation of Woody Plants (pp 425–442). Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar
  14. Lloyd G & McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latitulia, by use of shoot-tip culture. Comb. Proc. Int. Plant Prop. Soc. 30: 421–427Google Scholar
  15. Lu C-Y (1993) The use of thidiazuron in tissue culture. In Vitro Cell. Dev. Biol. 29P: 92–96Google Scholar
  16. Minocha SC (1987) Plant growth regulators and morphogenesis in cell and tissue culture of forest trees. In: Bonga JM & Durzan DJ (eds) Cell and Tissue Culture in Forestry, Vol I (pp 50–66). Martinus Nijhoff Publishers, Dordrecht, The NetherlandsGoogle Scholar
  17. Mok MC, Mok DWS & Armstrong DJ (1980) Cytokinin activity of N-phenyl-N0-1,2,3-thiadiazol-5-yl-urea and its effect on cytokinin autonomy in callus cultures of Phaseolus. Plant Physiol 65(suppl): 24 (Abstr)Google Scholar
  18. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
  19. Navarrete NE, Van Sambeek JW, Preece JE & Gaffeny GR (1989) Improved micropropagation of white ash (Fraxinus americana L.). In: Rink G & Budelsky CA (eds) Proceedings of the Seventh Central Hardwood Conference (pp 146–149). March 5-8. Carbondale, ILGoogle Scholar
  20. Preece JE, Christ PH, Ensenberger L & Zhao J (1987) Micropropagation of ash (Fraxinus). Comb. Proc. Intl. Plant Prop. Soc. 37: 366–372Google Scholar
  21. Preece JE, Zhao J & Kung FH (1989) Callus production and somatic embryogenesis of white ash. HortSci. 24: 377–380Google Scholar
  22. Preece JE, Huetteman CH, Ashby WC & Roth PL (1991) Microand cutting propagation of silver maple. II. Genotype and provenance affect performance. J.Amer. Soc. Hort. Sci. 116: 149–155Google Scholar
  23. Preece JE, Bates SA & Van Sambeek JW (1995) Germination of cut seeds and seedling growth of ash (Fraxinus spp.) in vitro. Can. J. For. Res. 25: 1368–1374Google Scholar
  24. Solomon JD, Leininger TD, Wilson AD, Anderson RL, Thompson LC & McCracken FI (1993) Ash pests: a guide to major insects, diseases, air pollution injury, and chemical injury. U. S. Department of Agriculture, Forest Service, Gen. Tech. Rep. SO-96, New Orleans, LA (45p)Google Scholar
  25. SAS Institute (1985) SAS/IML User's guide. 5.0 Edition. SAS Institute, Inc., Cary, NCGoogle Scholar
  26. SAS Institute (1996) SAS/STAT User's guide. 6.11 Edition. SAS Institute, Inc., Cary, NCGoogle Scholar
  27. van Nieuwkerk JP, Zimmerman RH & Fordham I (1986) Thidiazuron stimulation of apple shoot proliferation in vitro. HortSci. 21: 516–518Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Mee-Sook Kim
    • 1
  • Carol M. Schumann
    • 2
  • Ned B. Klopfenstein
    • 2
  1. 1.Department of Forestry, Fisheries, and Wildlife, 101 Plant IndustriesUniversity of NebraskaLincolnU.S.A
  2. 2.USDA Forest Service, Rocky Mountain Forest and Range Experiment Station, National Agroforestry CenterEast Campus-University of NebraskaLincolnU.S.A

Personalised recommendations