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A routine method to determine the chlorophyll a, pheophytin a and β-carotene contents of isolated Photosystem II reaction center complexes

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Abstract

The most simple way in which the stoichiometry of chlorophyll a, pheophytin a and β-carotene in isolated Photosystem II reaction center complexes can be determined is by analysis of the spectrum of the extracted pigments in 80% acetone. We present two different calculation methods using the extinction coefficients of the purified pigments in 80% acetone at different wavelengths. One of these methods also accounts for the possible presence of chlorophyll b. The results are compared with results obtained with HPLC pigment analysis, and indicate that these methods are suitable for routine determination of the pigment stoichiometry of isolated Photosystem II reaction center complexes.

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Authors and Affiliations

  1. Department of Physics and Astronomy, Institute of Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands

    Camiel Eijckelhoff & Jan P. Dekker

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  1. Camiel Eijckelhoff
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  2. Jan P. Dekker
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Eijckelhoff, C., Dekker, J.P. A routine method to determine the chlorophyll a, pheophytin a and β-carotene contents of isolated Photosystem II reaction center complexes. Photosynthesis Research 52, 69–73 (1997). https://doi.org/10.1023/A:1005834006985

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  • DOI: https://doi.org/10.1023/A:1005834006985

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