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Influence of sulfur-oxidizing bacteria on the budget of sulfate in Yugama crater lake, Kusatsu-Shirane volcano, Japan

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Sulfur-oxidizing bacteria, Thiobacillus thiooxidans, werefound in a highly acidic (pH = 1∼1.5) crater lake, Yugama,seasonally flowing streams and soil in the catchment area of thecrater. Thiobacillus ferrooxidans was also found in some ofthe streams but not in the lake itself. The lake water containsaqueous carbon dioxide, hydrogen sulfide, polythionates andelemental sulfur in suspension which are the substrates for thegrowth of the sulfur-oxidizing bacteria as no organic compoundsexcept for the microorganisms themselves were detected. Thebacteria isolated from the Yugama water preferred polythionatesin the following order: S4O6 2> S5O6 2> S6O6 2On the other hand,H2S was more rapidly consumed by the bacteria thanpolythionates which were followed by elemental sulfur. In thecase of test-tube incubation, the optimum pH of the solution forgrowth of the bacteria was between 1.0 and 1.5, and forcultivation in growth medium plates between 2.5 and 3.5. Thebacteria hardly proliferated at pH 0.5 or below. In accordancewith these characteristics of the bacteria, numbers of thebacteria in the surface Yugama crater lake water were at minimum(< a few cells/mL) in February and at maximum (106 cell/mL)in August. The bacterial activity changed in accordance with thesurface lake water temperature, but not necessarily with thevariations in H2S and polythionates concentrations of the lakewater. Based on the variation in sulfur isotope ratios of sulfateand experimentally determined rate of oxidation of H2S in thelake water, the sulfate production rate by the bacteria in thecatchment area and the lake were estimated to 9.5 and 8.4g/m2/day, respectively, during the period from 1988 to 1990when the volcanic activity at Yugama was at minimum. Also stream,hydrothermal, H2S-oxidated SO2 4-inputs and outputs byseepage and precipitation have been calculated as 4.1, 32, 0.56,36, and 1.2 ton/day, respectively.

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  1. Bosecker K (1984) Biodegradation of sulfur minerals and its application for metal recovery. In: Müller A & Krebs B (Eds) Sulfur (p 331). Elsevier, Amsterdam

  2. Bott TL & Brock TD (1969) Bacterial growth rates above 90 °C in Yellowstone Hot Springs. Science 164: 1411–1412

  3. Brock TD & Darland GK (1970) Limits of microbial existence: temperature and pH. Science 169: 1316–1318

  4. Delmelle P & Bernard A (1994) Geochemistry, mineralogy, and chemical modeling of the acid crater lake of Kawah Ijen Volcano, Indonesia. Geochim. Cosmochim. Acta 58: 2445–2460

  5. Hoefs J (1973) Stable Isotope Geochemistry (p 101). Springer Verlag, Berlin/Heidelberg/New York

  6. Garrels RM & Thompson ME (1960) Oxidation and pyrite in ferric sulfate solution. Am. J. Sci. 268: 57–67

  7. Ivanov MV & Karavaiko GI (1966) The role of microorganisms in the sulfur cycle in crater lakes of the Golovnin caldera. Zeits. Allg. Mikrobiol. 6: 10–22

  8. Jørgensen BB, Isaksen MF & Jannasch HW (1992) Bacterial sulfate reduction above 100 °C in deep-sea hydrothermal vent sediments. Science 258: 1756–1758

  9. Kaplan IR, Emery KO & Rittenberg SC (1963) The distribution and isotopic abundance of sulfur in recent marine sediments of Southern California. Geochim. Cosmochim. Acta 27: 297–331

  10. Kobayashi J (1960) A chemical study of the average quality and characteristics of river water of Japan. Ber. Ohara Inst. Landwirtshaft. Biol. Okayama Univ. 11: 313–357

  11. Konno H, Sasaki K, Tsunekawa M, Takamori T & Furuichi R (1991) X-ray photoelectron spectroscopic analysis of surface products on pyrite formed by bacterial leaching. Bunseki Kagaku (Japan Analyst) 40: 609–616 (in Japanese)

  12. Ljunggren P (1960) A sulfurmud deposit formed through bacterial transformation of fumarolic hydrogen sulfide. Econ. Geol. 55: 531–538

  13. Matsumoto G & Watanuki K (1992) Organic geochemical features of an extremely acid crater lake (Yugama) of Kusatsu-Shirane Volcano in Japan. Geochem. J. 26: 117–136

  14. McKibben MA & Barnes HL (1986) Oxidation of pyrite in low temperature acidic solutions: Rate laws and surface textures. Geochim. Cosmochim. Acta 50: 1509–1520

  15. Meyer B (1976) Elemental Sulfur. Chem. Rev. 76: 367–388

  16. Murozumi M, Abiko T & Nakamura S (1966) Geochemical investigation of the Noboribetsu Oyunuma explosion crater lake. Volcanol. Soc. Japan Bull., 2nd Ser. 11: 1–16

  17. Ohashi R (1919) On the peculiar sulfur spherules produced in a crater lake of the volcano Shirane, in the province of Kozuke, Central Japan. J. Akita Mining College (1): 1–10

  18. Ohba T, Hirabayashi J & Nogami K (1994)Water, heat and chloride budgets of the crater lake, Yugama at Kusatsu-Shirane volcano, Japan. Geochem. J. 28: 217–231.

  19. Ohsawa S (1992) Geochemical studies on the behavior of metastable sulfur compounds in solution-application to volcanology. Ph.D dissertation (Univ. Tokyo) (in Japanese)

  20. Ohsawa S, Takano B, Kusakabe M & Watanuki K (1993) Variation in volcanic activity of Kusatsu-Shirane volcano as inferred from δ34S in sulfate from the Yugama crater lake. Bull. Volcanol. Soc. Japan 38: 95–99

  21. Ozawa T (1966) Rapid determination of elemental sulfur by absorption spectrometry. Nippon Kagaku Zasshi. (J. Chem. Soc. Japan) 87: 578–580

  22. Pearson FJ Jr & Coplen TB (1978) Stable isotope studies of lakes. In: Lehman A (Ed) Lakes: Chemistry, Geology, Physics (pp 325–339). Springer Verlag, New York

  23. Rowe GL, Ohsawa S, Takano B, Brantley SL, Fernandez JF & Barquero J (1992a) Using crater lake chemistry to predict volcanic activity Nat Poás Volcano, Costa Rica. Bull. Volcanol. 54: 494–503

  24. Rowe GL, Brantley SL, Fernandez M, Fernandez JF, Borgia A & Barquero J (1992b) Fluidvolcano interaction in an active stratovolcano: The crater lake system of Poás volcano, Costa Rica.J. Volcanol. Geotherm. Res. 49: 23–51.

  25. Sakai H (1957) Fractionation of sulfur isotopes in nature. Geochim. Cosmochim. Acta 16: 574–577

  26. Sasaki K, Tsunekawa M & Konno H (1994) Nonstoichiometry in the oxidative dissolution of pyrite in acid solutions. Bunseki Kagaku (Japan Analyst) 43: 911–917 (in Japanese)

  27. Satake K & Saijo Y (1974) Carbon dioxide content and metabolic activity of microorganisms in some acid lakes in Japan. Limnol. Oceanogr. 19: 331–338

  28. Schoen R (1969) Rate of sulfuric acid formation in Yellowstone National Park. Geol. Soc. Am. Bull. 80: 643–650

  29. Schwartz A & Schwartz W (1979) Mikroorganismen und Lagerstätten-Entstehung in heißen Schwfelseen auf Hokkaido (Japan). Zeits. Allg. Mikrobiol. 19: 497–510

  30. Shimoya M(1985) The eruptions at Kusatsu-Shirane volcano. In: Shirane Kazan (p 49). Jomo Press, Maebashi

  31. 6 (n = 3~22) and their detection in cultures of Thiobacillus ferrooxidans; molecular composition of bacterial sulfur speciation. Angew}. Chem. Inst. Ed. Engl. 26: 151–153

  32. Sukawa A (1960) Evaporation from the water surface of high temperature. Geophys. Bull. Hokkaido Univ 7: 63–70 (in Japanese)

  33. Takano B (1987) Correlation of volcanic activity with sulfur oxyanion speciation in a crater lake. Science 235: 1633–1635

  34. Takano B & Watanuki K (1990) Monitoring of volcanic eruptions at Yugama crater lake by aqueous sulfur oxyanions. J. Volcanol. Geotherm. Res. 40: 71–87

  35. Takano B, Ohsawa S & Glover RB(1994a) Surveillance ofRuapehu Crater Lake, New Zealand, by aqueous polythionates. J. Volcanol. Geotherm. Res. 60: 29–57

  36. Takano B, Saitoh H & Takano, E (1994b) Geochemical implications of subaqueous molten at Yugama crater lake, Kusatsu-Shirane volcano, Japan. Geochem. J. 28: 199–216

  37. Togano T & Ochiai M(1987) Quantitative analysis of sulfide ions in hot spring waters. Kagaku to Kyoiku (Chem. Educ.) 35: 346–347 (in Japanese)

  38. Tsuya H (1933) Explosive activity of volcano Kusatu-Sirane in October, 1932. Bull. Earthq. Res. Inst. 11: 82–113

  39. Tuovinen OH & Kelly DP (1973) Studies on the growth of Thiobacillus ferrooxidansI. Use of membrane filter and ferrous ion agar to determine viable numbers and comparison with 14CO2-fixation and iron oxidation by means of growth. Archiv Mikrobiol. 88: 285–298

  40. Wakao N, Mishima M, Sakurai Y & Shiota H (1982) Bacterial pyrite oxidation I. The effect of pure and mixed cultures of Thiobacillus ferrooxidansand Thiobacillus thiooxidanson release of iron. J. Gen. Appl. Microbiol. 28: 331–343

  41. Webster JG (1989) An analytical scheme for the determination of sulfide, polysulfide, thiosulfate, sulfite and polythionate concentrations in geothermal waters. Report No. CD2406. Institute of Geological & Nuclear Sciences Limited, New Zealand.

  42. Wiesma CL & Rimstidt JD (1984) Rates of reaction of pyrite and marcasite with ferric iron at pH 2. Geochim. Cosmochim. Acta 48: 85–9

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TAKANO, B., KOSHIDA, M., FUJIWARA, Y. et al. Influence of sulfur-oxidizing bacteria on the budget of sulfate in Yugama crater lake, Kusatsu-Shirane volcano, Japan. Biogeochemistry 38, 227–253 (1997). https://doi.org/10.1023/A:1005805100834

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  • crater lake
  • polythionates
  • sulfate budget
  • sulfur-oxidizing bacteria
  • Thiobacillus ssp.