Abstract
The regeneration response of mature and immature rice embryos from 15 rice varieties (Oryza sativa L. var. indica) was separately measured in terms of regeneration per cent and total number of regenerated plantlets obtained for a fixed sample size per variety, when cultured on standard MS media. The response of the rice varieties was classed as high, medium or poor, based on the above criteria. The regeneration per cent of genotypes showing high, medium and poor response ranged from 67 to 97, 38 to 63 and 0 to 36 respectively. The variation in the regeneration response amongst the defined groups was found to be statistically significant in terms of regeneration per cent and total plantlets for a constant sample size when subjected to one way analysis of variance and Duncan's multiple range test. Only small influences on regeneration frequency were found when the most and least responsive genotypes, Binnatoa and BR-23 were cultured on N6 and MS media with additional or different supplements.
Similar content being viewed by others
References
Abe T and Futsuhara Y (1984) Varietal difference of plant regeneration from root callus tissues in rice. Jpn. J. Breed. 34: 147–155
Abe T & Futsuhara Y (1986) Genotypic variability for callus formation and plant regeneration in rice. Theor. Appl. Gen. 72: 3–10
Chan M-T, Lee T-M & Chang H-H (1992) Transformation of indica rice medicated by Agrobacterium tumefaciens. Plant Cell Physiol. 33: 577–583
Christou P, Ford TL & Kofron M (1992) The development of a variety independent gene transfer method for rice. Tibtech 10: 239–246
Datta SK, Datta K, Soltanifar N, Donn G & Potrykus I (1992) Herbicide-resistant Indica rice plants from IRRI breeding line IR72 after PEG-mediated transformation of protoplasts. Plant Mol. Biol. 20: 619–629
Faure O, Mengoli M, Nougarhde A & Bagni N (1991) Polyamine pattern and biosynthesis in zygotic and somatic embryo stages of Vitis vinifera. J. Plant Physiol. 138: 545–549
Ghosh Biswas GC, Iglesias VA, Datta SK & Potrykus I (1994) Transgenic Indica rice plants obtained by direct gene transfer to protoplasts. J. of Biotech 32: 1–10
Hiei Y, Ohta S, Komari T & Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.)mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal 6: 271–282
Kavi Kishor PBK & Reddy GM (1986) Regeneration of plants from long term cultures of Oryza sativa L. Plant Cell Reports 5: 391–393
Mikami T & Kinoshita T (1988) Genotypic effects on callus formation from different explants of rice. Plant Cell Tissue Organ Cult. 12: 311–314
Peng J, Kononowicz H & Hodges TK (1992) Transgenic Indica rice plants. Theor. Appl. Genet. 83: 855–863
Seraj ZI, Sarker AB & Islam AS (1992) Plant regeneration in a jute species (Corchorus capsularis) and its possible relationship with glyoxalase I. Plant Cell Reports 12: 29–33
van Engelen FA & de Vries SC (1992) Extracellular proteins in plant embryogenesis. Trends in Genetics 8: 66–70
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Seraj, Z.I., Islam, Z., Faruque, M.O. et al. Identification of the regeneration potential of embryo derived calluses from various Indica rice varieties. Plant Cell, Tissue and Organ Culture 48, 9–13 (1997). https://doi.org/10.1023/A:1005766513009
Issue Date:
DOI: https://doi.org/10.1023/A:1005766513009