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Plant Cell, Tissue and Organ Culture

, Volume 48, Issue 1, pp 37–44 | Cite as

Morphogenetic plasticity of callus reinitiated from cell suspension cultures of the fern Platycerium coronarium

  • Siew-Hwa Kwa
  • Yeow-Chin Wee
  • Tit-Meng Lim
  • Prakash P. Kumar
Article

Abstract

Cell suspension cultures were initiated from gametophyte-derived callus of the fern Platycerium coronarium. Two distinct types of callus masses, distinguished by their colouration, were obtained when the cells from suspension culture were plated on semisolid Murashige and Skoog (MS) medium containing 10 μM kinetin. The two types of callus masses had distinct morphogenetic capacities despite their common origin. Morphogenesis into either gametophytes or sporophytes occurred when these callus masses were cultured on phytohormone-free MS medium depending on the type of callus used. The dark-green gametophytic callus showed a faster rate of growth and morphogenesis as compared to the pale-green sporophytic callus. Total chlorophyll content and autofluorescence and size of chloroplasts of the sporophytic callus and cell suspension cultures were lower than that of the gametophytic callus. Observations from confocal laser scanning microscopy were in agreement with the physiological parameters measured. The availability of cell cultures of the same ploidy level, but with two distinct pathways of development will be useful for comparative studies of developmental plasticity.

cell suspension cultures confocal laser scanning microscopy gametophyte morphogenetic plasticity sporophyte Platycerium coronarium 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Siew-Hwa Kwa
    • 1
  • Yeow-Chin Wee
    • 1
  • Tit-Meng Lim
    • 1
  • Prakash P. Kumar
    • 1
  1. 1.School of Biological SciencesNational University of SingaporeSingapore

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