Biogeochemistry

, Volume 36, Issue 2, pp 153–172

Investigation of the methyl fluoride technique for determining rhizospheric methane oxidation

  • JOANNE E. LOMBARDI
  • MICHELLE A. EPP
  • JEFFREY P. CHANTON

Abstract

Methane oxidation rates in the rhizosphere of Pontederia cordata,Sagittaria lancifolia, and Typha latifolia were quantified in fieldstudies using the methyl fluoride inhibition technique. An averageoxidation of 22.9 ± 17.7% (sd,n = 44) was found for all field experiments (oxidation is expressedas a % of total potential emission in the presumedabsence of oxidation). Greenhouse experiments using the same techniquegave an average rhizospheric oxidation of 64.9 ±17.0% (sd, n = 44). Comparison of a subset ofgreenhouse plants with the methyl fluoride (MF) and a light oxic/darkanoxic (LO/DA) technique for suppressing CH4 oxidationyielded similar percentages (57.7 ±15.0% for MF and 58.5 ±13.9% for LO/DA, n = 11). Rhizospheric oxidationdisplayed a seasonal trend in Typha latifolia with decreasingoxidation percentages during warmer months as the importance ofrhizospheric CH4 oxidation declined relative toCH4 emission (46.5 ±13.8% in December and 13.5 ±1.7% in July). However, the absolute rateof methane oxidation was highest during the warmer months (44.2± 3.4 mg m-2 d-1 inDecember and 318.7 ± 151.4 mg m-2d-1 in July). As methane emission rates increased,the sensitivity of the methyl fluoride technique decreased dueto the larger error between replicate flux measurements.

aquatic marcrophytes methane methane oxidation methyl fluoride plant/microbial interactions rhizosphere 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • JOANNE E. LOMBARDI
    • 1
  • MICHELLE A. EPP
    • 2
  • JEFFREY P. CHANTON
    • 2
  1. 1.Department of ChemistryFlorida State UniversityTallahassee
  2. 2.Department of OceanographyFlorida State UniversityTallahassee
  3. 3.Institute of Marine SciencesUniversity of AlaskaFairbanks

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