Plant Molecular Biology

, Volume 33, Issue 6, pp 1093–1096 | Cite as

Identification and characterization of (GA/CT) n - microsatellite loci from Quercus petraea

  • H. Steinkellner*
  • S. Fluch
  • E. Turetschek
  • C. Lexer
  • R. Streiff
  • A. Kremer
  • K. Burg
  • J. Glössl


In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-locus pattern. The number of alleles found per locus varied from 6 to 16. Combining the genetic variation observed for the characterized loci provides a unique genotype for all the individuals tested. Using intraspecific controlled crosses of Q. robur trees Mendelian inheritance could be shown for five loci.

genetic marker simple sequence repeat microsatellite oak genome Quercus 


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  1. 1.
    Akkaya MS, Bhagwat AA, Cregan PB: Length polymorphisms of simple sequence repeat DNA in soybeans. Genetics 132: 1131–1139 (1992).Google Scholar
  2. 2.
    Armour JAL, Neumann R, Gobert S, Jeffreys AJ: Isolation of human simple repeat loci by hybridization selection. Hum Mol Genet 3: 599–605 (1994).Google Scholar
  3. 3.
    Bennett MD, Smith JB: NuclearDNAamounts in angiosperms. Phil Trans R Soc Lond B 334: 309–346 (1991).Google Scholar
  4. 4.
    Broun P, Tanksley SD: Characterization and genomic mapping of simple repeat sequences in tomato genome. Mol Gen Genet 250: 39–49 (1996).Google Scholar
  5. 5.
    Condit R, Hubbell SP: Abundance and DNA sequence of twobase repeat regions in tropical tree genomes. Genome 34: 66–71 (1991).Google Scholar
  6. 6.
    Dow BD, Ashley MV, Howe HF: Characterization of highly variable (GA/TC)n microsatellites in the bur oak, Quercus macrocarpa. Theor Appl Genet 91: 137–141 (1995).Google Scholar
  7. 7.
    Echt CS, May Marquardt P, Hseih M, Zahorchak R: Characterization ofmicrosatellite markers in eastern white pine. Genome (in press).Google Scholar
  8. 8.
    John B, Miklos GLG: The Eukaryote Genome in Development and Evolution. Allen and Unwin, London (1988).Google Scholar
  9. 9.
    Lagercrantz U, Ellegren H, Andersson L: The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates. Nucl Acids Res 21: 1111–1115 (1993).Google Scholar
  10. 10.
    Litt M, Lutty JA: A hypervariable microsatellite revealed by in vitro amplification of dinucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet 44: 397–401 (1989).Google Scholar
  11. 11.
    Kijas JMH, Fowler JCS, Thomas M: An evaluation of sequence tagged microsatellite site markers for genetic analysis with Citrus and related species. Genome 38: 349–355 (1995)Google Scholar
  12. 12.
    Kostia S, Varvio S-L, Vakkari P, Pulkkinen P: Microsatellite sequence in a conifer, Pinus sylvestris. Genome 38: 1244–1248 (1995).Google Scholar
  13. 13.
    Morgante M, Olivieri AM: PCR-amplified microsatellites as markers in plant genetics. Plant J 3: 175–182 (1993).Google Scholar
  14. 14.
    Saghai-Maroof MA, Biyaswhev RM, Yang GP, Zhang Q, Allard RW: Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 91: 5499–5470 (1994).Google Scholar
  15. 15.
    Schmidt T, Heslop-Harrison JS: The physical and genomic organization of microsatellites in sugar beet. Proc Natl Acad Sci USA 93: 8761–8765 (1996).Google Scholar
  16. 16.
    Smith DN, Devey ME: Occurence and inheritance of microsatellites in Pinus radiata. Genome 37: 977–983 (1994).Google Scholar
  17. 17.
    Stallings RL, Ford AF, Nelson D, Torney DC, Hildebrand CE, Moyzis RK: Evolution and distribution of (GT)n repetitive sequences in mammalian genomes. Genomics 10: 807–815 (1991).Google Scholar
  18. 18.
    Taramino G, Tingey S: Simple sequence repeats for germplasm analysis and mapping in maize. Genome 39: 277–287 (1996).Google Scholar
  19. 19.
    Terauchi R: A polymorphic microsatellite marker from the tropical tree Dryobalanops lanceolata (Dipterocarpaceae). Jpn J Genet 69: 567–576 (1994).Google Scholar
  20. 20.
    Thomas MR, Scott NS: Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequencetagged sites (STSs). Theor Appl Genet 86: 985–990 (1993).Google Scholar
  21. 21.
    Valdes AM, Slatkin M, Freimer NB: Allele frequencies at microsatellite loci: the stepwise mutation model revisited. Genetics 133: 737–749 (1993).Google Scholar
  22. 22.
    Wang Z, Weber JL, Zhong G, Tanksley SD: Survey of plant short tandem DNA repeats. Theor Appl Genet 88: 1–6 (1994).Google Scholar
  23. 23.
    Weber JL: Informativeness of human (dC-dA)n/(dG-dT)n polymorphism. Genomics 7: 524–530 (1990).Google Scholar
  24. 24.
    Yang GP, Saghai-Maroof MA, Xu CG, Zhang Q, Biyaswhev RM: Comparative analysis of microsatellite DNA polymorphism in landraces and cultivars of rice. Mol Gen Genet 245: 187–195 (1994).Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • H. Steinkellner*
    • 1
  • S. Fluch
    • 1
    • 2
  • E. Turetschek
    • 1
  • C. Lexer
    • 1
  • R. Streiff
    • 3
  • A. Kremer
    • 3
  • K. Burg
    • 2
  • J. Glössl
    • 1
  1. 1.Zentrum für Angewandte GenetikUniversität für Bodenkultur WienViennaAustria
  2. 2.Forschungszentrum SeibersdorfSeibersdorfAustria
  3. 3.Station de Recherches Forestieres de Bordeaux-Cestas, PierrotonINRACestasFrance

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