Biotechnology Letters

, Volume 22, Issue 11, pp 941–945

One-step partial purification of Solanum tuberosum tuber lectin using magnetic chitosan particles

  • Mirka Šafaříková
  • Ivo Šafařík


A one-step procedure for a partial purification of Solanum tuberosum tuber lectin has been developed. Lectin from tuber extract or from potato wastewater was adsorbed to magnetic chitosan particles and eluted with low pH buffer. The specific activity of separated lectin increased 13 times during the purification process and the recovery was 50%.

chitosan lectin magnetic particles Solanum tuberosum 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bendikene VG, Juodka BA, Kazlauskas RM, Tautkus CA, Matulionis EA, Sudavichius AA (1995) Immobilization of enzymes on carriers with magnetic properties. Preparation and characterization of magnetic derivatives of chitin. Appl. Biochem. Microbiol. 31: 335–340.Google Scholar
  2. Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.Google Scholar
  3. Dessai NN, Allen AK (1979) The purification of potato lectin by affinity chromatography on an N-,N'-,N”-riacetylchitotriose-Sepharose matrix. Anal. Biochem. 93: 88–90.Google Scholar
  4. Honda H, Kawabe A, Shinkai A, Kobayashi T (1998) Developmentof chitosan-conjugated magnetite for magnetic cell separation. J. Ferment. Bioeng. 86: 191–196.Google Scholar
  5. Kopáček P, Vogt R, Jindrák L, Weise C, Šafařík I (1999) Purification and characterization of the lysozyme from the gut of the oft tick Ornithodoros moubata. Insect Biochem. Mol. Biol. 29: 989–997.Google Scholar
  6. Owens RJ, Northcote DH (1980) The purification of potato lectin by affinity chromatography on fetuin-Sepharose matrix. Phytochemistry 19: 1861–1862.Google Scholar
  7. Šafařík I (1991) Magnetic biospecific affinity adsorbents for lysozyme isolation. Biotechnol. Tech. 5: 111–114.Google Scholar
  8. Šafařík I (1995) Removal of organic polycyclic compounds from water solutions with a magnetic chitosan based sorbent earing copper phthalocyanine dye. Water Res. 29: 101–105.Google Scholar
  9. Šafařík I, Šafaříková M (1993) Batch isolation of hen egg white lysozyme with magnetic chitin. J. Biochem. Biophys. Meth. 27: 327–330.Google Scholar
  10. Šafařík I, Šafaříková M (1997) Overview of magnetic separations used in biochemical and biotechnological applications. In: Häfeli U, Schütt W, Teller J, Zborowski M, eds. Scientific and Clinical Applications of Magnetic Carriers. New York: Plenum Press, pp. 323–340.Google Scholar
  11. Šafařík I, Šafaříková M (1999) Use of magnetic techniques for the isolation of cells. J. Chromatogr. B 722: 33–53.Google Scholar
  12. Šafaříková M, Nymburská K, Blažek Z, Šafařík I (1996) Rapid removal of magnetic particles from large volumes of suspensions. Biotechnol. Tech. 10: 391–394.Google Scholar
  13. Wierzba-Arabska E, Morawiecka B (1987) Purification and properties of lectin from potato tubers and leaves; interaction with acid phosphatase from potato tuber. Acta Biochim. Pol. 34: 407–420.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Mirka Šafaříková
    • 1
  • Ivo Šafařík
    • 1
  1. 1.Laboratory of Biochemistry and MicrobiologyInstitute of Landscape Ecology

Personalised recommendations