Biotechnology Letters

, Volume 22, Issue 16, pp 1311–1317

A new efficient expression system for Bacillus and its application to production of recombinant phytase

  • Janne Kerovuo
  • Niklas von Weymarn
  • Mira Povelainen
  • Sanna Auer
  • Andrei Miasnikov
Article

DOI: 10.1023/A:1005694731039

Cite this article as:
Kerovuo, J., von Weymarn, N., Povelainen, M. et al. Biotechnology Letters (2000) 22: 1311. doi:10.1023/A:1005694731039

Abstract

A new expression system was developed for Bacillus subtilis.This system uses a shuttle vector (B. subtilisEschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillusphytase – phytase activity at the end of cultivation reached 28.7 U ml−1.

Bacillus subtilis fed-batch cultivation phosphate-starvation phytase plasmid 

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Janne Kerovuo
    • 1
  • Niklas von Weymarn
    • 2
  • Mira Povelainen
    • 3
  • Sanna Auer
    • 3
  • Andrei Miasnikov
    • 3
  1. 1.Danisco Cultor InnovationKantvik Research CentreFinland
  2. 2.Laboratory of Bioprocess EngineeringHelsinki University of TechnologyEspooFinland
  3. 3.Danisco Cultor InnovationKantvik Research CentreKantvikFinland

Personalised recommendations