A new efficient expression system for Bacillus and its application to production of recombinant phytase
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- Kerovuo, J., von Weymarn, N., Povelainen, M. et al. Biotechnology Letters (2000) 22: 1311. doi:10.1023/A:1005694731039
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A new expression system was developed for Bacillus subtilis.This system uses a shuttle vector (B. subtilis– Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillusphytase – phytase activity at the end of cultivation reached 28.7 U ml−1.