Biotechnology Letters

, Volume 22, Issue 11, pp 931–936 | Cite as

Enhancement of chrysogenin production in cultures of Penicillium chrysogenum by uronic acid oligosaccharides

  • E. Asilonu
  • C. Bucke
  • T. Keshavarz


Additions of 50 to 100 μg of acid-hydrolysed alginate oligosaccharides ml−1 and enzyme-hydrolysed pectin oligosaccharides to 24- to 48-h cultures of Penicillium chrysogenum, ATCC 9480, led to enhanced production of chrysogenin by over 30 to 40% in shaken flasks and bioreactors. Some of the oligosaccharides also promoted biomass formation but were not used as a carbon source.

chrysogenin elicitors enhancement oligosaccharides Penicillium chrysogenum 


  1. Albersheim P, Darvill A, McNeil M, Valent BS, Sharp JK, NothnagelEA, Davis KR, Yamazaki N (1983) Oligosaccharins: naturallyoccurring carbohydrates with biologic regulatory functions.In: Ciferri, C, Dure L,eds. Structure and Functions of PlantGenomes. New York: Plenum Press, pp. 293–312.Google Scholar
  2. Ariyo B, Bucke C, Keshavarz T (1997) Alginate oligosaccharidesas enhancers of penicillin production in cultures of Penicilliumchrysogenum. Biotech. Bioeng. 53: 17–20.Google Scholar
  3. Braun DM, Walker JC (1996) Plant transmembrane receptors: newpieces in the signaling puzzle. Trend Biotechnol. 21: 70–73.Google Scholar
  4. Cluttterbuck PW, Lovell R, Raistrick H (1932) Studies in the biochemistryof microorganisms. The formation from glucose bymembers of the Penicillin chrysogenum series of a pigment,an alkaline soluble protein and penicillin – the antibacterial substance of Fleming. Biochem. J. 26: 1907–1919.Google Scholar
  5. Eilert U (1987) Elicitation: methodology and aspects of application.In: Vasil IK, Constabel F, eds. Cell Culture and Somatic Cel lGenetics of Plants, Vol. 4. San Diego: Academic Press, pp. 153–196.Google Scholar
  6. Haug A, Larsen B, Smidsrød O (1966) A study of the constitution of alginic acid by partial acid hydrolysis. Acta. Chem. Scand. 20:183–190.Google Scholar
  7. Marero LM, Je Hyeong J, Joong HS, Ho JL, In Sik C, Hyong JL (1997) Effect of elicitation on indirubin production from asuspension culture of Polygonum tinctorium. Enzyme Microbiol.Tech. 21: 97–101.Google Scholar
  8. Polne-fuller M, Amano A, Fujimura T (1993) Enhanced developmentand differentiation of protoplasts and spores of green andred seaweeds by Pterocladia agar from New Zealand. Hydrobiologia 261: 499–504.Google Scholar
  9. Skjak-Braek G, Espevik T (1996) Application of alginate gels inbiotechnology and biomedicine. Carbohydrates in Europe 14:19–25.Google Scholar
  10. Suvarnalatha G, Rajendra L, Ravishankar GA, Venkataraman LV(1994) Elicitation of anthocyanin production in cell cultures ofcarrot Daucus carota using elicitors and abiotic stress. Biotechnol.Lett. 16: 1275–1280.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • E. Asilonu
    • 1
  • C. Bucke
    • 1
  • T. Keshavarz
    • 1
  1. 1.Fungal Biotechnology Group, Biotechnology DepartmentUniversity of WestminsterLondon UK

Personalised recommendations