Journal of Muscle Research & Cell Motility

, Volume 21, Issue 7, pp 609–620 | Cite as

R403Q and L908V mutant β-cardiac myosin from patients with familial hypertrophic cardiomyopathy exhibit enhanced mechanical performance at the single molecule level

  • Kimberly A. Palmiter
  • Matthew J. Tyska
  • Joe R. Haeberle
  • Norman R. Alpert
  • Lameh Fananapazir
  • David M. Warshaw
Article

Abstract

Familial hypertrophic cardiomyopathy (FHC) is a disease of the sarcomere. In the β-myosin heavy chain gene, which codes for the mechanical enzyme myosin, greater than 40 point mutations have been found that are causal for this disease. We have studied the effect of two mutations, the R403Q and L908V, on myosin molecular mechanics. In the in vitro motility assay, the mutant myosins produced a 30% greater velocity of actin filament movement (νactin). At the single molecule level, νactind/ton, where d is the myosin unitary step displacement and ton is the step duration. Laser trap studies were performed at 10 μM MgATP to estimate d and ton for the normal and mutant myosin molecules. The increase in νactin can be explained by a significant decrease in the average ton's in both the R403Q and L908V mutants (∼30 ms) compared to controls (∼40 ms), while d was not different for all myosins tested (∼7 nm). Thus the mutations affect the kinetics of the cross-bridge cycle without any effect on myosin's inherent motion and force generating capacity. Based on these studies, the primary signal for the hypertrophic response appears to be an apparent gain in function of the individual mutant myosin molecules.

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Kimberly A. Palmiter
    • 1
  • Matthew J. Tyska
    • 1
  • Joe R. Haeberle
    • 1
  • Norman R. Alpert
    • 1
  • Lameh Fananapazir
    • 2
  • David M. Warshaw
  1. 1.Department of Molecular Physiology and BiophysicsUniversity of VermontBurlington
  2. 2.Division of CardiologyNational Institutes of HealthBethesda

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