Journal of Muscle Research & Cell Motility

, Volume 21, Issue 2, pp 171–181 | Cite as

S-NO-actin: S-nitrosylation kinetics and the effect on isolated vascular smooth muscle

  • Isabella Dalle-donne
  • Aldo Milzani/snm>
  • Daniela Giustarini
  • Paolo Di Simplicio
  • Roberto Colombo
  • Ranieri Rossi
Article

Abstract

We describe the modification of reactive actin sulfhydryls by S-nitrosoglutathione. Kinetics of S-nitrosylation and denitrosylation suggest that only one cysteine of actin is involved in the reactions. By using the bifunctional sulfhydryl cross-linking reagent N,N′-1,4-phenylenebismaleimide and the monofunctional reagent N-iodoacetyl-N′-(5-sulpho-1-naphthyl)ethylenediamine, we identified this residue as Cys374. The time course of filament formation followed by high-shear viscosity changes revealed that S-nitrosylated G-actin polymerizes less efficiently than native monomers. The observed decrease in specific viscosity at steady state is due mainly to a marked inhibition of filament end-to-end annealing and, partially, to a reduction in F-actin concentration. Finally, S-nitrosylated actin acts as nitric oxide donor showing a fast, potent vasodilating activity at unusually low concentrations, being comparable with that of low molecular weight nitrosothiols.

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Isabella Dalle-donne
    • 1
  • Aldo Milzani/snm>
    • 1
  • Daniela Giustarini
    • 2
  • Paolo Di Simplicio
    • 2
  • Roberto Colombo
    • 1
  • Ranieri Rossi
    • 2
  1. 1.Lab. Biochem. Biophys. Cytoskel., Department of BiologyUniversity of MilanMilanItaly
  2. 2.Pharmacology Section, Institute for Mental and Nervous DiseasesUniversity of SienaSienaItaly

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