Biotechnology Letters

, Volume 22, Issue 7, pp 623–629

Engineering the Escherichia coli outer membrane protein OmpC for metal bioadsorption

  • Norberto Cruz
  • Sylvie Le Borgne
  • Georgina Hernández-Chávez
  • Guillermo Gosset
  • Fernando Valle
  • Francisco Bolivar
Article

Abstract

The outer membrane protein, OmpC, from Escherichia coli was used to display metal-binding poly-histidine peptides on the surface of this bacterium. SDS-PAGE analysis of outer membrane protein preparations confirmed the expression of the metal-binding epitopes inserted in position 162 of the mature OmpC protein. Display of these epitopes was confirmed by epifluorescence microscopy of cells bound to Ni2+-NTA-agarose beads and metal adsorption experiments. The cells harboring one or two copies of the metal binding epitope were able to adsorb 3 to 6 times more Zn2+ (13.8 μmol g−1 cell), Fe3+ (35.3 μmol g−1 cell), and Ni2+ (9.9 μmol g−1 cell) metallic ions than control cells expressing the wild-type OmpC.

Escherichia coli genetic engineering metal-binding OmpC protein engineering 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Norberto Cruz
    • 1
  • Sylvie Le Borgne
    • 1
    • 1
  • Georgina Hernández-Chávez
    • 1
    • 1
  • Guillermo Gosset
    • 1
    • 1
  • Fernando Valle
    • 1
    • 1
  • Francisco Bolivar
    • 1
  1. 1.Departamento de Microbiología Molecular, Instituto de BiotecnologíaUniversidad Nacional Autónoma de MexicoCuernavaca, MorelosMexico

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