Biotechnology Letters

, Volume 21, Issue 11, pp 997–1002 | Cite as

Genetic transformation via particle bombardment of Catharanthus roseus plants through adventitious organogenesis of buds

  • Rafael Zárate
  • Johan Memelink
  • Robert van der Heijden
  • Robert Verpoorte
Article

Abstract

In vitro propagation of Catharanthus roseus was achieved using nodal explants. Bud induction was best on medium containing 1.0 mg benzyl aminopurine l−1. Hardening of rooted shoots to soil was very successful with 98% survival. Genetically transformed C. roseus plantlets were obtained after bombardment of nodal explants, which were then micropropagated, with DNA coated particles with green fluorescent protein (GFP) or β-glucuronidase (GUS) reporter genes. Histological studies showed that the gene insertion method proved effective with many cells and different tissues displaying the reporter gene signals, showing that gene expressions were rather stable.

β-glucuronidase Catharanthus roseus genetic transformation green fluorescent protein particle bombardment 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Rafael Zárate
    • 1
  • Johan Memelink
    • 2
  • Robert van der Heijden
    • 1
  • Robert Verpoorte
    • 1
  1. 1.Division of Pharmacognosy, Gorlaeus laboratories, Leiden/Amsterdam Center for Drug Research (LACDR)Leiden UniversityRA LeidenThe Netherlands
  2. 2.Institute of Molecular Plant Sciences, Clusius LaboratoryLeiden UniversityAL LeidenThe Netherlands

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