Enhanced ajmalicine production in Catharanthus roseus cell cultures by combined elicitor treatment: from shake-flask to 20-l airlift bioreactor
- Cite this article as:
- Zhao, J., Zhu, WH. & Hu, Q. Biotechnology Letters (2000) 22: 509. doi:10.1023/A:1005616920165
A two-stage process for enhanced ajmalicine production in elicited Catharanthus roseus cell cultures was developed in shake-flasks and a bioreactor. By using combined elicitor treatment of an Aspergillum niger mycelium and tetramethyl ammonium bromide, yields of ajmalicine were 48 mg l−1, 52 mg l−1 and 33 mg l−1, respectively in 500-ml flasks, 1000-ml flasks and a 20-l airlift bioreactor. The peroxidase and superoxide dismutase activities decreased in elicited cell cultures but catalase and lipoxygenase activities increased in these cultures. The combined elicitor treatment also caused a significant increase of malondialdehyde content in cell cultures.