Biotechnology Letters

, Volume 22, Issue 6, pp 509–514

Enhanced ajmalicine production in Catharanthus roseus cell cultures by combined elicitor treatment: from shake-flask to 20-l airlift bioreactor

  • Jian Zhao
  • Wei-Hua Zhu
  • Qiu Hu
Article

DOI: 10.1023/A:1005616920165

Cite this article as:
Zhao, J., Zhu, WH. & Hu, Q. Biotechnology Letters (2000) 22: 509. doi:10.1023/A:1005616920165

Abstract

A two-stage process for enhanced ajmalicine production in elicited Catharanthus roseus cell cultures was developed in shake-flasks and a bioreactor. By using combined elicitor treatment of an Aspergillum niger mycelium and tetramethyl ammonium bromide, yields of ajmalicine were 48 mg l−1, 52 mg l−1 and 33 mg l−1, respectively in 500-ml flasks, 1000-ml flasks and a 20-l airlift bioreactor. The peroxidase and superoxide dismutase activities decreased in elicited cell cultures but catalase and lipoxygenase activities increased in these cultures. The combined elicitor treatment also caused a significant increase of malondialdehyde content in cell cultures.

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Jian Zhao
    • 1
  • Wei-Hua Zhu
    • 1
  • Qiu Hu
    • 1
  1. 1.Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R. China

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