PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli
- Cite this article as:
- Solaiman, D.K. Biotechnology Letters (2000) 22: 789. doi:10.1023/A:1005614209342
- 118 Downloads
A ca. 5.5-kb region of Pseudomonas resinovorans genome containing the polyhydroxyalkanoate (PHA) biosynthesis locus was sequenced. Three complete open-reading-frames (ORFs), i.e., phaC1Pr, phaZPr, and phaC2Pr, were identified. Using this sequence information, phaC1Pr was PCR-cloned from P. resinovorans genomic DNA and expressed in E. coli as shown by a Nile Red plate assay and gas chromatography/mass spectrometric analysis.