Biotechnology Letters

, Volume 22, Issue 9, pp 789–794

PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli

  • Daniel K.Y. Solaiman

DOI: 10.1023/A:1005614209342

Cite this article as:
Solaiman, D.K. Biotechnology Letters (2000) 22: 789. doi:10.1023/A:1005614209342


A ca. 5.5-kb region of Pseudomonas resinovorans genome containing the polyhydroxyalkanoate (PHA) biosynthesis locus was sequenced. Three complete open-reading-frames (ORFs), i.e., phaC1Pr, phaZPr, and phaC2Pr, were identified. Using this sequence information, phaC1Pr was PCR-cloned from P. resinovorans genomic DNA and expressed in E. coli as shown by a Nile Red plate assay and gas chromatography/mass spectrometric analysis.

gene expression PCR cloning pha genes polyhydroxyalkanoates Pseudomonas resinovorans 

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Daniel K.Y. Solaiman
    • 1
  1. 1.Eastern Regional Research CenterUS Department of Agriculture, ARSWyndmoorUSA

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