Advertisement

Biotechnology Letters

, Volume 22, Issue 6, pp 495–498 | Cite as

A hyper-thermostable, alkaline lipase from Pseudomonas sp. with the property of thermal activation

  • Pooja Rathi
  • Sapna Bradoo
  • R.K. Saxena
  • Rani Gupta
Article

Abstract

A hyper-thermostable, alkaline lipase from a newly-isolated, mesophilic Pseudomonas sp. was optimal at pH 11 and at 90 °C. It had a half-life of more than 13 h at 90 °C. It was activated by 30% when heated at 90 °C for 2 h. The enzyme had a greater affinity for mustard oil (Km=40 mg ml−1) than for olive oil (Km=140 mg ml−1).

hyper-thermostability lipase mustard oil Pseudomonas sp. thermal activation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ghosh PK, Saxena RK, Gupta R, Yadav RP, Davidson WS (1996) Microbial lipases: production and applications. Sci. Prog. 79: 119–157.Google Scholar
  2. Gilbert EJ (1993) Pseudomonas lipases: biochemical properties and molecular cloning. Enzyme Microbiol. Tech. 15: 634–645.Google Scholar
  3. Jaeger K-E, Ransac S, Dijkstra BW, Colson C, van Heuvel M, Misset O (1994) Bacterial lipases. FEMS Microbiol. Rev. 15: 29–63.Google Scholar
  4. Klump H, Di-Ruggiero J, Kessel M, Park JB, Adams MWW, Robb FT (1992) Glutamate dehydrogenase from the hyperthermophile Pyrococcus furiosus. J. Biol. Chem. 267: 22681–22685.Google Scholar
  5. Kulkarni N, Gadre RV (1999) A novel alkaline, thermostable, protease-free lipase from Pseudomonas sp. Biotechnol. Lett. 21: 897–899.Google Scholar
  6. Lee SY, Rhee JS (1993) Production and partial purification of a lipase from Pseudomonas putida 3SK. Enzyme Microbiol. Tech. 15: 617–623.Google Scholar
  7. Nahas E (1988) Control of lipase production by Rhizopus oligosporus under various growth conditions. J. Gen. Microbiol. 134: 227–233.Google Scholar
  8. Nawani N, Dosanjh NS, Kaur J (1998) A novel thermostable lipase from a thermophilic Bacillus sp.: characterization and esterification studies. Biotechnol. Lett. 20: 997–1000.Google Scholar
  9. Nishio T, Chikano T, Kamimura M (1987) Purification and some properties of lipase produced by Pseudomonas fragi 22.39B. Agric. Biol. Chem. 51: 181–186.Google Scholar
  10. Taipa MA, Aires-Barros MR, Cabral JMS (1992) Purification of lipases. J. Biotechnol. 26: 111–142.Google Scholar
  11. Vulfson EN (1994) Industrial application of lipases. In: Woolley P, Petersen SB, eds. Lipases: Their Structure, Biochemistry and Application. Cambridge: Cambridge University Press, pp. 271–288.Google Scholar
  12. Watanabe N, Ota Y, Minoda Y, Yamada K (1977) Isolation and identification of alkaline lipase producing microorganisms, cultural conditions and some properties of crude enzymes. Agric. Biol. Chem. 41: 1353–1358.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Pooja Rathi
    • 1
  • Sapna Bradoo
    • 1
  • R.K. Saxena
    • 1
  • Rani Gupta
    • 1
  1. 1.Department of MicrobiologyUniversity of DelhiNew DelhiIndia

Personalised recommendations