A hyper-thermostable, alkaline lipase from Pseudomonas sp. with the property of thermal activation
A hyper-thermostable, alkaline lipase from a newly-isolated, mesophilic Pseudomonas sp. was optimal at pH 11 and at 90 °C. It had a half-life of more than 13 h at 90 °C. It was activated by 30% when heated at 90 °C for 2 h. The enzyme had a greater affinity for mustard oil (Km=40 mg ml−1) than for olive oil (Km=140 mg ml−1).
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