Immobilization of invertase via carbohydrate moiety on chitosan to enhance its thermal stability
A new technique using chitosan as support for covalent coupling of invertase via carbohydrate moiety improved the activity and thermal stability of immobilized invertase. The best preparation of immobilized invertase retained 91% of original specific activity (412 U mg−1). The half-life at 60 °C was increased from 2.3 h (free invertase) to 7.2 h (immobilized invertase). In contrast, the immobilization of invertase via protein moiety on chitosan or using Sepharose as support resulted in less thermostable preparations. Additionally, immobilization of invertase on both supports caused the optimal reaction pH to shift from 4.5 to 2.5 and the substrate (sucrose) concentration for maximum activity to increase from 0.5 M to 1.0 M.
Unable to display preview. Download preview PDF.
- Carrara CR, Rubiolo AC (1994) Immobilization of β-galactosidase on chitosan. Biotechnol. Prog. 10: 220–224.Google Scholar
- Godbole SS, Kubal BS, D'Souza SF (1990) Hydrolysis of concentrated sucrose syrups by invertase immobilized on an ion exchanger waste cotton thread. Enzyme Microbiol. Tech. 12: 214–217.Google Scholar
- Husain S, Jafri F, Saleemuddin M (1996) Effects of chemical modification on the stability of invertase before and after immobilization. Enzyme Microbiol. Tech. 18: 275–280.Google Scholar
- Iqbal J, Saleemuddin M (1983) Activity and stability of glucose oxidase and invertase immobilized on concanavalin A-Sepharose. Biotechnol. Bioeng. 25: 3191–3195.Google Scholar
- Kauss H, Jeblick W, Domard A (1989) The degrees of polymerization and N-acetylation of chitosan determine its ability to elicit callus formation in suspension cells and protoplasts of Catharanthus roseus. Planta 178: 385–392.Google Scholar
- Madyastha KM, Ganguli AR, Kubair VG, Kowser N, Vidya D (1987) Extracellular invertase from Aspergillus athecius: isolation and immobilization. Biotechnol. Lett. 9: 555–560.Google Scholar
- Mansfeld J, Schellenberger A, Rombach J (1992) Application of polystyrene-bound invertase to continuous sucrose hydrolysis on pilot scale. Biotechnol. Bioeng. 40: 997–1003.Google Scholar
- Marek M, Valentova O, Kas J (1984) Invertase immobilization via its carbohydrate moiety. Biotechnol. Bioeng. 26: 1223–1226.Google Scholar
- Riccardo AA, Muzzarelli AA (1980) Immobilization of enzymes on chitin and chitosan. Enzyme Microbiol. Tech. 2: 177–184.Google Scholar
- Spagna G, Andreani F, Salatelli EE, Romagnoli D, Pifferi PG (1998) Immobilization of α-L-arabinofuranosidase on chitin and chitosan. Process Biochem. 33: 57–62.Google Scholar