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Digestive Diseases and Sciences

, Volume 45, Issue 7, pp 1439–1443 | Cite as

Binding of Hydrogen Sulfide by Bismuth Does Not Prevent Dextran Sulfate-Induced Colitis in Rats

  • Julie K. Furne
  • Fabrizis L. Suarez
  • Stephen L. Ewing
  • John Springfield
  • Michael D. Levitt
Article

Abstract

Several lines of evidence suggest that ulcerative colitis could be caused by excessive bacterial production of H2S in the colon. A rodent model of colitis involves the feeding of nonabsorbable, carbohydrate-bound sulfate in the form of dextran sulfate or carrageenan. The observation that metronidazole blocks the development of this colitis suggested that the injurious agent could be a sulfur-containing compound (such as H2S) that is released during the bacterial metabolism of the nonabsorbed sulfate. We tested this possibility by feeding rats dextran sulfate, with or without bismuth subsalicylate, a compound that avidly binds H2S. Bismuth subsalicylate reduced the fecal release of H2S in dextran sulfate-treated rats to values well below that of controls. Nevertheless, all the animals developed colitis. We conclude that excessive H2S production does not play a role in the dextran sulfate model of colitis.

hydrogen sulfide bismuth colitis rats 

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REFERENCES

  1. 1.
    Suarez FL, Fume JK, Springfield J, Levitt MD: Insights into human colonic physiology obtained from study of flatus composition. Am J Physiol 272:G1028-G1033, 1997Google Scholar
  2. 2.
    Gibson GR, Macfarlane GT, Cummings JH: Sulphate reducing bacteria and hydrogen metabolism in the human large intestine. Gut 34:437–439, 1993Google Scholar
  3. 3.
    Macfarlane GT, Gibson GR, Cummings JH: Comparison of fermentation reactions in different regions of the human colon. J Appl Bacteriol 72:57–64, 1992Google Scholar
  4. 4.
    Roediger WEW, Moore J, Babidge W: Colonic sulfide in pathogenesis and treatment of ulcerative colitis. Dig Dis Sci 42:1571–1579, 1997Google Scholar
  5. 5.
    Roediger WEW: The colonic epithelium in ulcerative colitis: An energy-deficiency disease? Lancet 2:712–715, 1980Google Scholar
  6. 6.
    Roediger WEW, Lawson MJ, Kwok V, Kerr Grant A, Pannall PR: Colonic bicarbonate output as a test of disease activity in ulcerative colitis. J Clin Pathol 37:704–707, 1984Google Scholar
  7. 7.
    Florin T, Neale G, Cummings J: The effect of hydrogen sulphide on isolated rat colonic epithelium. In Inflammatory Bowel Diseases. Progress in Basic Research and Clinical Implications. H Goebell, K Ewe, M Malchow, Koelbel CH (eds). Dordrecht, The Netherlands, Kluwer Academic, 1991, p 423Google Scholar
  8. 8.
    Ng W, Tonzetich J: Effect of hydrogen sulfide and methylmercaptan on the permeability of oral mucosa. J Dent Res 63:994–997, 1984Google Scholar
  9. 9.
    McKay LF, Eastwood MA, Brydon WG: Methane excretion in man-a study of breath, flatus, and faeces. Gut 26:69–74, 1985Google Scholar
  10. 10.
    Peled Y, Weinberg D, Hallak A, Gilat T: Factors affecting methane production in humans. Gastrointestinal diseases and alterations of colonic flora. Dig Dis Sci 32:267–271, 1987Google Scholar
  11. 11.
    Florin THJ, Gibson GR, Neale G, Cummings JH: A role for sulfate reducing bacteria in ulcerative colitis? Gastroenterology 98:A170, 1990Google Scholar
  12. 12.
    Gibson GR, Cummings JH, Macfarlane GT: Growth and activities of sulphate-reducing bacteria in gut contents of healthy subjects and patients with ulcerative colitis. FEMS Microbiol Ecol 86:103–112, 1991Google Scholar
  13. 13.
    Levine J, Ellis CJ, Furne JK, Springfield J, Levitt MD: Fecal hydrogen sulfide production in ulcerative colitis. Am J Gastroenterol 93:83–87, 1998Google Scholar
  14. 14.
    Okayasu I, Hatakeyama S, Yamada M, Ohdusa T, Inagadi Y, Nakaya R: A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology 98:694–702, 1990Google Scholar
  15. 15.
    Marcus NJ, Marcus SN, Marcus R, Watt J: Rapid production of ulcerative disease of the colon in newly-weaned guinea-pigs by degraded carrageenan. J Pharm Pharmacol 41:423–426, 1989Google Scholar
  16. 16.
    Ohkusa T, Yamada M, Takenaga T, Kitazume C, Yamamoto N, Sasabe M, Takashimizu I, Tamura Y, Sakamoto E, Kurosawa H, Fujimoto H, Nishiura M, Nakamura R, Kuyama Y, Aoki N, Inagaki Y, Chida T, Higaki M, Okayasu I, Hatakeyama S: Protective effect of metronidazole in experimental ulcerative colitis induced by dextran sulfate sodium. Nippon Shokakibyo Gakkai Zasshi 84:2337–2346, 1987Google Scholar
  17. 17.
    Suarez FL, Fume JK, Springfield J, Levitt MD: Bismuth subsalicylate markedly decreases hydrogen sulfide release in the human colon. Gastroenterology 114:923–929, 1998Google Scholar

Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Julie K. Furne
    • 1
  • Fabrizis L. Suarez
    • 1
  • Stephen L. Ewing
    • 1
  • John Springfield
    • 1
  • Michael D. Levitt
    • 1
  1. 1.Research ServiceMinneapolis VA Medical CenterMinneapolis

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