Journal of Chemical Ecology

, Volume 26, Issue 7, pp 1667–1683 | Cite as

Metapleural Gland Secretion of the Leaf-cutter Ant Acromyrmex octospinosus: New Compounds and Their Functional Significance

  • Diethe Ortius-Lechner
  • Roland Maile
  • E. David Morgan
  • Jacobus J. Boomsma


Ants of the myrmicine tribe Attini live in symbiosis with a fungus that provides them with food. In return the ants maintain optimal growth conditions for the fungus, weed out competing microorganisms, and inhibit the growth conditions of these competitors by chemical means. We present a comprehensive analysis of metapleural gland secretions of Acromyrmex octospinosus, using a recently developed method for the analysis of polar compounds by gas chromatography and mass spectrometry. We show that the chemical identity and quantitative recovery of different compounds in the metapleural gland secretion depends upon the method used and the type of colony from which the samples are taken. In addition to the two compounds previously recorded in the metapleural gland secretion of Acromyrmex ants (indolacetic acid and myrmicacin), 20 new compounds were detected in the secretion of a random sample of workers from two laboratory colonies and two field colonies. These compounds span the whole range of carboxylic acids from acetic acid to the long-chain fatty acids but comprise also some alcohols, lactones, and keto acids. The possible function of this highly complex secretion mixture is discussed.

Formicidae leaf-cutter ants Acromyrmex octospinosus gas chromatography mass spectrometry metapleural gland carboxylic and fatty acids lactones keto acids antibiotics 


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  1. Attygalle, A. B., Siegel, B., Vostowsky, O., Bestmann, H. J., and Maschwitz, U. 1989. Chemical composition and function of metapleural gland secretion of the ant, Crematogaster deformis Smith (Hymenoptera: Myrmicinae). J. Chem. Ecol. 15:317–328.Google Scholar
  2. Bass, M., and Cherrett, J. M. 1994. The role of leaf-cutting ant workers (Hymenoptera: Formicidae) in fungus garden maintenance. Ecol. Entomol. 19:215–220.Google Scholar
  3. Beattie, A. J., Turnbull, C., Hough, T., Jobson, S., and Knox, R. B. 1985. The vulnerability of pollen and fungal spores to ant secretions: Evidence and some evolutionary implications. Am. J. Bot. 72(4):606–614.Google Scholar
  4. Beattie, A. J., Turnbull, C., Hough, T., and Knox, R. B. 1986. Antibiotic production: A possible function for the metapleural glands of ants (Hymenoptera: Formicidae). Ann. Entomol. Soc. Am. 79:448–450.Google Scholar
  5. Blum, M. S. 1981. Chemical Defenses of Arthropods. Academic Press, New York, 562 pp.Google Scholar
  6. Bot, A. N. M., and Boomsma, J. J. 1996. Variable metapleural gland size-allometries in Acromyrmex leafcutter ants (Hymenoptera: Formicidae). J. Kans. Entomol. Soc. 69:375–383.Google Scholar
  7. Boyd, N. D., and Martin, M. M. 1975. Faecal proteinases of the fungus-growing ant Atta texana: Properties, significance and possible origin. Insect Biochem. 5:619–635.Google Scholar
  8. Brough, E. J. 1983. The antimicrobial activity of the mandibular gland secretion of a formicine ant, Calomyrmex sp. (Hymenoptera: Formicidae). J. Invert. Pathol. 42:306–311.Google Scholar
  9. Brown, W. L. 1968. An hypothesis concerning the function of the metapleural glands in ants. Am. Nat. 102:188–191.Google Scholar
  10. Cherrett, J. M., Powell, R. J., and Stradling, D. J. 1989. The mutualism between leaf-cutting ants and their fungus, pp. 93–120. Insect-Fungus Interactions. Royal Entomological Society of London, 14th Symposium, 1987, Academic Press, London.Google Scholar
  11. Cowles, P. B. 1941. The germicidal action of the hydrogen ion and of the lower fatty acids. Yale J. Biol. Med. 13:571–578.Google Scholar
  12. Craven, S. E., Dix, M. W., and Michaels, G. E. 1970. Attine fungus gardens contain yeasts. Science 169:184–186.Google Scholar
  13. Currie, C. R., Scott, J. A., Summerbell, R. C., and Malloch, D. 1999a. Fungus-growing ants use antibiotic-producing bacteria to control garden parasites. Nature 398:701–704.Google Scholar
  14. Currie, C. R., Mueller, U. G., and Malloch, D. 1999b. The agricultural pathology of ant fungus gardens. Proc. Natl. Acad. Sci. U.S.A. 96:7998–8002.Google Scholar
  15. Dettner, K. 1984. Isopropylesters as wetting agents from the defensive secretion of the rove beetle Coprophilus striatulus F. (Coleoptera, Staphylinidae). Insect Biochem. 14:383–390.Google Scholar
  16. Diehl-Fleig, and Valim-Labres, M. E. 1993. Fungi isolated from leaf-cutting ants Atta sexdens piriventris and Acromyrmex heyeri (Hymenoptera: Formicidae): Mucor spp. effects on Beauveria bassiana entomopathogen. Cienc. Cult. 45:142–144.Google Scholar
  17. do Nascimento, R. R., Schoeters, E., Morgan, D. E., Billen, J., and Stradling, D. 1996. Chemistry of metapleural gland secretions of three attine ants, Atta sexdens rubropilosa, Atta cephalotes and Acromyrmex octospinosus (Hymenoptera: Formicidae). J. Chem. Ecol. 22:987–1000.Google Scholar
  18. Dowd, P. F. 1992. Insect fungal symbionts: A promising source of detoxifying enzymes. J. Ind. Microbiol. 9:149–161.Google Scholar
  19. Eisner, T., Meinwald, J., Monro, A., and Ghent, R. 1961. Defense mechanism of arthropods—I. The composition and function of the spray of the whipscorpion, Mastigoproctus giganteus (Lucas) (Arachnida, Pedipalpida). J. Insect Physiol. 6:272–298.Google Scholar
  20. Fisher, P. J., Stradling, D. J., Sutton, B., and Petrini, C. L. E. 1996. Microfungi in the fungus gardens of the leaf-cutting ant Atta cephalotes: A preliminary study. Mycol. Res. 100:541–546.Google Scholar
  21. Hervey, A., and Nair, M. S. R. 1979. Antibiotic metabolites of a fungus cultured by the gardening ants. Mycologia 71:1064–1066.Google Scholar
  22. HÖlldobler, B., and Engel-Siegel, H. 1984. On the metapleural gland in ants. Psyche 91:201–224.Google Scholar
  23. Iwanawi, Y. 1978. Myrmicacin, a new inhibitor for mitotic progression after metaphase. Protoplasma 95:267–271.Google Scholar
  24. JaffÉ, K., and Puche, H. 1984. Colony-specific territorial marking with the metapleural gland secretion in the ant Solenopsis geminata (Fabr.). J. Insect Physiol. 30:265–270.Google Scholar
  25. Knapp, J. J., Jackson, C. W., Howse, P. E., and Vilela, E. F. 1994. Mandibular gland secretions of leaf-cutting ants: Role in defence against alien fungi, p. 109, in A. Lenoir, G. Arnold, and M. Lepage (eds.). Les Insectes Sociaux, 12th Congress of the International Union for the Study of Social Insects, Paris, Université Paris.Google Scholar
  26. Koidsumi, K. 1957. Antifungal action of cuticular lipids in insects. J. Insect Physiol. 1:40–51.Google Scholar
  27. Kreisel, H. 1972. Pilze aus Pilzgärten von Atta insularis in Kuba. Z. Allg. Mikrobiol. 12:643–654.Google Scholar
  28. Maile, R., Dani, F. R., Jones, G. R., Morgan, E. D., and Ortius, D. 1998. Sampling techniques for gas chromatographic-mass spectrometric analysis of long-chain free fatty acids from insect exocrine glands. J. Chromatogr. A 816:169–175.Google Scholar
  29. Maschwitz, U. 1974. Vergleichende Untersuchungen zur Funktion der Ameisenmetathorakaldrüse. Oecologia 16:303–310.Google Scholar
  30. Maschwitz, U., Koob, K., and Schildknecht, H. 1970. Ein Beitrag zur Funktion der Metathorakaldr üse der Ameisen. J. Insect. Physiol. 16:387–404.Google Scholar
  31. Maurer, P., Debieu, D., Malosse, C., Leroux, P., and Riba, G. 1992. Sterols and symbiosis in the leaf-cutting ant Acromyrmex octospinosus (Reich) (Hymenoptera, Formicidae: Attini). Arch. Insect Biochem. Physiol. 20:13–21.Google Scholar
  32. Morgan, E. D. 1990. Preparation of small-scale samples from insects for chromatography. Anal. Chim. Acta 236:227–235.Google Scholar
  33. North, R. D., Jackson, C. W., and Howse, P. E. 1997. Evolutionary aspects of ant-fungus interactions in leaf-cutting ants. Tree 12:386–389.Google Scholar
  34. Papa, J., and Papa, F. 1982. Inhibition des bacteries dans les nids d'Acromyrmex octospinosus Reich. Bull. Soc. Pathol. Exotique 75:415–425.Google Scholar
  35. Pavan, M. 1958. Significato chimico e biologica di alcuni veleni di insetti. Inst. Anat. Comp. Univ. Pavia 1–75.Google Scholar
  36. Powell, R. J., and Stradling, D. J. 1986. Factors influencing the growth of Attamyces bromatificus, a symbiont of attine ants. Trans. Br. Mycol. Soc. 87:205–213.Google Scholar
  37. Quinlan, R. J., and Cherrett, J. M. 1977. The role of substrate preparation in the symbiosis between the leaf-cutting ant Acromyrmex octospinosus (Reich) and its food fungus. Ecol. Entomol. 2:161–170.Google Scholar
  38. Rice, R. W. 1989. Analyzing tables of statistical tests. Evolution 43(1):223–225.Google Scholar
  39. Schildknecht, H., and Koob, K. 1971. Myrmicacin, the first insect herbicide. Angew. Chem. 10:124–125.Google Scholar
  40. Schoeters, E., and Billen, J. 1993. Anatomy and fine structure of the metapleural gland in Atta (Hymenoptera, Formicidae). Belg. J. Zool. 123:67–75.Google Scholar
  41. Spoehr, H. A., Smith, J. H. C., Strain, H. H., Milner, H. W., and Hardin, G. J. 1949. Fatty Acid Antibacterials from Plants. Carnegie Institution of Washington Publications, Washington, D.C., pp. 36–68.Google Scholar
  42. Veal, D. A., Trimble, J. E., and Beattie, A. J. 1992. Antimicrobial properties of secretions from the metapleural glands of Myrmecia gulosa (the Australian bull ant). J. Appl. Bacteriol. 72:188–194.Google Scholar
  43. Weber, N. A. 1966. Fungus-growing ants. Science 153:587–604.Google Scholar
  44. Wyss, O., Ludwig, B. J., and Joiner, R. R. 1945. The fungistatic and fungicidal action of fatty acids and related compounds. Arch. Biochem. 7:415–425.Google Scholar

Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Diethe Ortius-Lechner
    • 1
  • Roland Maile
    • 3
  • E. David Morgan
    • 3
  • Jacobus J. Boomsma
    • 1
  1. 1.Department of Ecology and GeneticsUniversity of Aarhus, Ny MunkegadeAarhus CDenmark
  2. 2.Lehrstuhl für Biologie IUniversität RegensburgRegensburgGermany
  3. 3.Chemical Ecology GroupSchool of Chemistry and Physics, KeeleStaffordshireEngland

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