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Biotechnology Letters

, Volume 20, Issue 5, pp 483–488 | Cite as

Cloning and Sequencing of the nrdF Gene of Corynebacterium Ammoniagenes ATCC 6872 Encoding the Functional Metallo-Cofactor of the Manganese-Ribonucleotide Reductase (Mn-RRase)

  • W. Oehlmann
  • U. Griepenburg
  • G. Auling
Article

Abstract

Cloning of a 4.3 kb DNA fragment from Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 allowed sequencing and homologous expression of the nrdF gene encoding the metallo-cofactor (CA2 protein) of the Mn-RRase. Due to a multi copy effect transconjugants of C. ammoniagenes showed a sixfold increased resistance towards the radical-scavenger hydroxyurea (HU). The organic radical in the purified CA2 protein generated a visible absorption at 437 nm, absent upon manganese-depletion, and a distinct fluorescence at 513 nm. The two signals were each quenched by HU.

Keywords

Organic Chemistry Bioorganic Chemistry Hydroxyurea Organic Radical Corynebacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • W. Oehlmann
    • 1
  • U. Griepenburg
    • 1
  • G. Auling
    • 1
  1. 1.Institut für MikrobiologieUniversität HannoverHannoverGermany

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