Tropical Animal Health and Production

, Volume 32, Issue 5, pp 267–276 | Cite as

Comparison between Comparative Tuberculin and Gamma-Interferon Tests for the Diagnosis of Bovine Tuberculosis in Ethiopia

  • Gobena Ameni
  • Hakan Miörner
  • François Roger
  • Markos Tibbo


A study to determine and compare the sensitivities and specificities of the comparative cervical tuberculin (CCT) and gamma-interferon (IFN-γ) tests for the diagnosis of bovine tuberculosis was conducted on 30 zebu oxen. The results of the tests were compared with the presence of acid-fast bacilli found by bacteriological culturing and histopathological examinations. The sensitivity and specificity of CCT test were found to be 90.9% and 100%, respectively. Those of the commercial IFN-γ test were determined to be 95.5% and 87.7%, respectively. No significant differences were found between the sensitivities (Yates' corrected χ2 = 0.32; p = 0.57) or the specificities (Yates' corrected χ2 = 2.54; p = 0.11) of the two tests. Furthermore, a positive correlation (r = 0.76) was recorded between the increase in skin thickness following injection of bovine purified protein derivative (PPD) and the optical density in the gamma-interferon assay with bovine PPD. On the other hand, the correlation (r = 0.47) between the change in skin thickness following injection of avian PPD and the optical density in the gamma-interferon assay with avian PPD was relatively weak. On the basis of this preliminary investigation, it was concluded that the choice between the two tests depends on their cost and simplicity and on livestock management and time factors rather than on their respective diagnostic value.

bovine tuberculosis cattle diagnosis gamma-interferon Mycobacterium PPD tuberculin test 


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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Gobena Ameni
    • 1
  • Hakan Miörner
    • 2
  • François Roger
    • 3
  • Markos Tibbo
    • 4
  1. 1.Institute of Pathobiology and Faculty of Veterinary MedicineAddis Ababa UniversityAddis AbabaEthiopia
  2. 2.Armauer Hansen Research InstituteAddis AbabaEthiopia
  3. 3.CIRAD-EMVT, Campus International de BaillarguetMontpellierFrance
  4. 4.International Livestock Research Institute (ILRI), AnGRAddis AbabaEthiopia

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