Advertisement

Plant and Soil

, 223:245 | Cite as

Copper uptake and translocation in chicory (Cichorium intybus L. cv Grasslands Puna) and tomato (Lycopersicon esculentum Mill. cv Rondy) plants grown in NFT system. II. The role of nicotianamine and histidine in xylem sap copper transport

  • M. T. Liao
  • M. J. Hedley
  • D. J. Woolley
  • R. R Brooks
  • M. A. Nichols
Article

Abstract

The effect of rooting media Cu concentration (0.05–20 mg Cu L-1) on amino acid concentrations and copper speciation in the xylem sap of chicory and tomato plants was measured using 6 week old plants grown in a nutrient film technique system (NFT). Irrespective of the Cu concentration in the nutrient solutions, more than 99.68% and 99.74% of total Cu in tomato and chicory xylem sap was in a bound form. When exposed to high Cu concentrations in the rooting media, amino acid concentrations in the sap increased. Relative to other amino acids, the concentrations of glutamine (Gln), histidine (His), asparagine (Asn), valine (Val), nicotianamine (NA) and proline (Pro) in tomato xylem saps, and His, γ-aminobutyric acid (Gaba), glutamic acid (Glu), leucine (Leu), NA and phenylalanine (Phe) in chicory xylem saps showed the greatest increases. The data indicate that induced synthesis of some free amino acids as a specific and proportional response to Cu treatment. For a single complexation amino acid, the solution Cu2+concentration vs pH titration curve for NA at 0.06–0.07 mM was most similar, closely followed by His at 0.5–0.6 mM, to the solution Cu2+concentration behaviour in both tomato and chicory xylem sap. It is concluded that increased Cu concentrations in the rooting media induced selective synthesis of certain amino acid which include NA, His, Asn and Gln which have high stability constants with Cu. NA and His have the highest binding constants for Cu and the concentrations of NA and His in chicory and tomato xylem saps can account for all the bound Cu carried in the sap.

amino acids chicory copper tomato xylem sap 

References

  1. Beneš I, Schreiber K, Ripperger H and Kircheiss A 1983 Metal complex formation by nicotianamine, a possible phytosiderophore. Exper. 39, 261–262.CrossRefGoogle Scholar
  2. Budêšínský M, Budzikiewiez H, Procházka Z, Ripperger H, Römer A, Scholz G and Schreiber K 1980 Nicotianamine, a possible phytosiderophore of general occurrence. Phytochem. 19, 2295–2297.CrossRefGoogle Scholar
  3. Fekkes D 1996 State-of-the-art of high-performance liquid chromatographic analysis of amino acids in physiological samples. J. Chromatogr. B Biomed. Appl. 682, 3–22.PubMedCrossRefGoogle Scholar
  4. Fierabracci V, Masiello P, Novelli M and Bergamin E 1991 Application of amino acid analysis by high-performance liquid chromatography with phenyl isothiocyanate derivatization to the rapid determination of free amino acids in biological samples. J. Chromatogr. 570, 285–291.PubMedGoogle Scholar
  5. Graham R D 1979 Transport of copper and manganese to the xylem exudate of sunflower. Plant Cell Environ. 2, 139–143.CrossRefGoogle Scholar
  6. Griffin R A and Jurinak J J 1973 Estimation of activity coefficients from the electrical conductivity of natural aquatic systems and soil extracts. Soil Sci. 116, 26–30.Google Scholar
  7. Krämer U, Cotter-Howells J D, Charnock J M, Baker A J M and Smith J A C 1996 Free histidine as a metal chelator in plants that accumulate nickel. Na. 379, 635–638.Google Scholar
  8. Krijger G C, Van Vliet P M and Wolterbeen H T 1999 Metal speciation in xylem exudate of Lycopersion esculentum Mill-technetium. Plant Soil 212, 165–173.CrossRefGoogle Scholar
  9. Liao M T, Hedley M J, Woolley D J, Brooks R R and Nichols M A 1999 Effect of amino acids and casein on Cu uptake by chicory from soil. Proc.NZ Grassl Assoc. 61, 181–184.Google Scholar
  10. Liao M T, Hedley M J, Woolley D J, Brooks R R and Nichols M A 2000 Copper uptake and translocation in chicory (Cichorium intybus L. cv. Grasslands Puna) and tomato (Lycopersicon esculentum Mill. cv. Rondy) plants grown in NFT system. I. Copper uptake and distribution in plants. Plant Soil (Accepted, PLSO8769).Google Scholar
  11. Loneragan J F 1981 Distribution and movement of copper in plants. In Copper in Soils and Plants. Eds. JF Loneragan, AD Robson and RD Graham. pp 165–188. Academic Press, New York.Google Scholar
  12. May P M, Linder PW and Williams D R 1977 Computer simulation of metal-ion equilibria in biofluids: Models for the low-molecular-weight complex distribution of calcium (II), magnesium (II), manganese (II), iron (III), copper (II), Zinc (II) and lead (II) ions in human blood plasma. J. Chem. Soc. 13, 588–595.Google Scholar
  13. Noma M and Noguchi M 1976 Occurrence of nicotianamine in higher plants. Phytochem. 15, 1701–1702.CrossRefGoogle Scholar
  14. Noma M, Noguchi M and Tamaki E 1971 A new amino acid, nicotianamine, from tobacco leaves. Tetrahedron Lett. 22, 2017–2020.CrossRefGoogle Scholar
  15. Pate J S 1976 Nutrients and metabolites of fluids recovered from xylem and phloem: Significance in relation to long-distance transport in plants. In Transport and Transfer Processes in Plants. Eds. IF Wardlaw and JB Passioura. pp 253–281. Academic Press, New York.Google Scholar
  16. Pich A and Scholz G 1996 Translocation of copper and other micronutrients in tomato plants (Lycopersicon esculentum Mill.): Nicotianamine-stimulated copper transport in the xylem. J. Exp. Bot. 47, 41–47.Google Scholar
  17. Pich A, Scholz g and Stephan Udo W 1994 Iron-dependent changes of heavy metals, nicotianamine and citrate in different plant organs and in the xylem exudate of two tomato genotypes. Nicotianamine as possible copper translocator. Plant Soil 165, 189–196.CrossRefGoogle Scholar
  18. SAS Institute, Inc. 1987 SAS user's guide: Statistics. Version 6.04. SAS Institute, Inv., Cary, NC.Google Scholar
  19. Scholz G, Faust J, Ripperger H and Schreibe, K 1988 Structurefunction relationships of nicotianamine analogues. Phytochem. 27, 2749–2754.CrossRefGoogle Scholar
  20. Senden M H M N, Meer Van der A J G M, Limborgh J and Wolterbeek H T H 1992 Analysis of major tomato xylem organic acids and PITC-derivatives of amino acids by RP-HPLC and UV detection. Plant Soil 142, 81–89.Google Scholar
  21. Stephan U W, Schmidke I, Stephan V W and Scholz G 1996 The nicotianamine molecule is made-to-measure for complexation of metal micronutrients in plants. BioMetals 9, 84–90.CrossRefGoogle Scholar
  22. Stephan U Wand Scholz G 1993 Nicotianamine: mediator of transport of iron and heavy metals in the phloem? Physiol. Plant. 88, 522–529.CrossRefGoogle Scholar
  23. Sugiura Y and Nomoto K 1984 Phytosiderophores: Structures and Properties of Mugineic Acids and Their Metal Complexes. Struct. Bonding 58, 106–135.Google Scholar
  24. Tiffin L O 1972 Translocation of micronutrients in plants. In Micronutrients in Agriculture. Eds. JJ Mortvedt, PM Giordano, and WL Lindsay. pp 199–229. American Society of Agronomy. Madison.Google Scholar
  25. Walker C D and Webb J 1981 Copper in plants: forms and behaviour. In Copper in Soils and Plants. Eds. Loneragan J F, Robson A D and Graham R D. pp 189–212. Academic Press, New York.Google Scholar
  26. Welch R M 1995 Micronutrient nutrition of plants. Crit Rev. Plant Sci. 14, 49–82.Google Scholar
  27. White M C, Baker F D Chaney R L and Decker A M 1981b Metal complexation in xylem fluid. II. Theoretical equilibrium model and computational computer program. Plant Physiol. 67, 301–310.PubMedGoogle Scholar
  28. White MC, Decker AMand Chaney R L 1981a Metal complexation in xylem fluid. I. Chemical composition of tomato and soybean stem exudate. Plant Physiol. 67, 292–300.PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • M. T. Liao
  • M. J. Hedley
  • D. J. Woolley
  • R. R Brooks
  • M. A. Nichols

There are no affiliations available

Personalised recommendations