Plant and Soil

, Volume 223, Issue 1–2, pp 33–46 | Cite as

Estimating crop N uptake from organic residues using a new approach to the 15N isotope dilution technique

  • Rebecca Hood
  • Roel Merckx
  • Erik Steen Jensen
  • David Powlson
  • Mirta Matijevic
  • Gudni Hardarson


Experiments were conducted to test a new approach to the 15N isotope dilution technique for estimating crop N uptake from organic inputs. Soils were pre-labelled with 15N fertiliser and a carbon source. These were then incubated until there was stabilisation of the 15N abundance of the inorganic N pool and resumption of inorganic N concentrations. Residues were then applied to the soils and planted with ryegrass (Lolium perenneL.) to determine the nitrogen derived from the residue (Ndfr) using the isotope dilution equations. This method was compared with the direct method, i.e. where 15N-labelled residues were added to the soil and Ndfr in the ryegrass calculated directly. Estimates of percentage nitrogen derived from the residue (%Ndfr) alfalfa (Medicago sativaL.) in the ryegrass, were similar, 22 and 23% for the direct and soil pre-labelling methods, respectively, in the Wechsel sandy loam. Also, estimates of the %Ndfr from soybean (Glycine max (L.) Merr) residues in the Krumbach sandy loam were similar 34% (direct) and 36% (soil pre-labelling approach). However, in the Seibersdorf clay loam, the %Ndfr from soybean was 49% using the direct method and 61% using the soil pre-labelling method; yet Ndfr from common bean residue was 46% using the direct approach and 40% using the pre-labelling, not significantly different (P > 0.05). The soil pre-labelling approach appears to give realistic values for Ndfr. It was not possible to obtain an estimate of Ndfr using the soil pre-labelling method from the maize residues (Zea mays L.) in two of the soils, as there was no increase in the total N of the ryegrass over the growing period. This was probably due to microbial immobilisation of inorganic N, as a result of the wide C:N ratio of the residue added. The results suggest that the new soil pre-labelling method is feasible and that it is a potentially useful technique for measuring N release from a wide range or organic residues, but it requires further field-testing.

crop residues nitrogen organic residues 


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  1. Alexander M 1977 Introduction to Soil Microbiology. 2nd Edn. pp 242–250. John Wiley and Sons, New York.Google Scholar
  2. Brookes P D, Stark J M, Mc Inteer B B and Preston T 1989 Diffusion method to prepare soil extracts for automated nitrogen-15 analysis. Soil Sci. Soc. Am. J. 53, 1707–1711.CrossRefGoogle Scholar
  3. Hadas A, Feigenbaum S, Sofer M, Molina J A E and Clapp C E 1993 Decomposition of nitrogen-15-labelled wheat and cellulose in soil: modelling tracer dynamics. Soil Sci. Soc. Am. J. 57, 996–1001.CrossRefGoogle Scholar
  4. Hart P B S, Rayner J H and Jenkinson D S 1986 Influence of pool substitution on the interpretation of fertilizer experiments with 15N. J. Soil Sci. 37, 389–403.Google Scholar
  5. Hauck R D and Bremner J M 1976 Use of tracers for soil and fertiliser nitrogen research. Adv. Agron. 28, 219-266.CrossRefGoogle Scholar
  6. He D Y, Liao X L, Xing T X, Zhou W J, Fang Y J and He L H 1994 The fate of nitrogen from 15N labelled straw and green manure in soil-crop domestic animal systems. Soil Sci. 158, 65–73.Google Scholar
  7. Hewitt E J 1966 Sand and water culture methods used in the study of plant nutrition. pp 430–451. Commonwealth Agricultural Bureaux, England.Google Scholar
  8. Hood R C, N'Goran K, Aigner Mand Hardarson G 1999 A comparison of direct and indirect 15N isotope techniques for estimating crop N uptake from organic residues. Plant Soil 208, 259-270.CrossRefGoogle Scholar
  9. Hu S, Grunwald N J, Van Bruggen A H C, Gamble G R, Drinkwater L E, Shennan C and Demment M W 1997 Short term effects of cover crop incorporation on soil carbon pools and nitrogen availability. Soil Sci. Soc. Am. J. 61, 901–911.CrossRefGoogle Scholar
  10. Jenkinson D S, Fox R H and Rayner J H 1985 Interactions between fertilizer nitrogen and soil nitrogen - the so called ‘priming’ effect. J. Soil Sci. 36, 425–444.Google Scholar
  11. Jensen E S, Andersen A J and Thomsen J D 1985 The influence of seed-borne N in 15N dilution studies with legumes. Acta Agric. Scand. 35, 438–443.CrossRefGoogle Scholar
  12. Kumarasinghe K S and Eskew D L 1993 Comparison of direct and indirect 15N methods for evaluation of N uptake by rice from Azolla. In Isotopic Studies of Azolla and Nitrogen Fertilization of Rice. Eds. K S Kumarasinghe and D L Eskew. pp 16–21. Kluwer Academic Publishers, Dordrecht, The Netherlands.Google Scholar
  13. Ladd J N, Oades J M and Amato M 1981 Microbial biomass from 14C, 15N-labelled plant material decomposing in soils in the field. Soil Biol. Biochem. 13, 119–126.CrossRefGoogle Scholar
  14. McAuliffe C, Chamblee D S, Uribe-Arango H and Woodhouse W W Jr. 1958 Influence of inorganic nitrogen on nitrogen fixation as revealed by 15N. Agron. J. 50, 334–337.CrossRefGoogle Scholar
  15. McNeill A M, Hood R C and Wood M 1994 Direct measurement of nitrogen fixation by Trifolium repens L. and Alnus glutinosa L. using 15N2. J. Exp. Bot. 75, 749–755.Google Scholar
  16. Melillo J M, Aber J D and Muratore J F 1982 Nitrogen and lignin control of hardwood leaf litter decomposition dynamics. Ecology 63, 621–626.CrossRefGoogle Scholar
  17. Powlson D S and Barraclough D 1993 Mineralization and assimilation in soil-plant systems. In Nitrogen Isotope Techniques. Eds. R Knowles and T H Blackburn. pp 209–242. Academic Press Inc, San Diego.Google Scholar
  18. Seligman N G, Feigenbaum S, Feinerman D and Benjamin R W 1986 Uptake of nitrogen from high C-N ratio, 15N-labelled organic residues by spring wheat grown under semi-arid conditions. Soil Biol. Biochem. 18, 303–307.CrossRefGoogle Scholar
  19. Senaratne R and Hardarson G 1988 Estimation of the residual N effect of faba bean and pea on two succeeding cereals using 15N methodology. Plant Soil 110, 81-89.CrossRefGoogle Scholar
  20. Sørensen P and Jensen E S 1998 The use of 15N labelling to study the turnover and utilization of ruminant manure. Biol. Fertil. Soils 28, 56–63.CrossRefGoogle Scholar
  21. Sørensen P, Jensen E S and Nielsen N E 1994a. Labelling animal manure nitrogen with 15N. Plant Soil 162, 31–37.CrossRefGoogle Scholar
  22. Sørensen P, Jensen E S and Nielsen N E 1994b. The fate of 15N labelled organic nitrogen in sheep manure applied to soils of different texture under field conditions. Plant Soil 162, 39–37.CrossRefGoogle Scholar
  23. Stevenson F C, Walley F L and Van Kessel C. 1998 Direct vs. indirect nitrogen-15 approaches to estimate nitrogen contributions from crop residues. Soil. Sci. Am. J. 62, 1327–1334.CrossRefGoogle Scholar
  24. Watkins N and Barraclough D 1996 Gross rates of N mineralization associated with the decomposition of plant residues. Soil Biol. Biochem. 28, 169–175.CrossRefGoogle Scholar
  25. Witty J F and Ritz K 1984 Slow release 15N fertiliser formulations to measure N2 fixation by isotope dilution. Soil Biol. Biochem. 16, 657–661.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Rebecca Hood
    • 1
  • Roel Merckx
    • 2
  • Erik Steen Jensen
    • 3
  • David Powlson
    • 4
  • Mirta Matijevic
    • 5
  • Gudni Hardarson
    • 5
  1. 1.Soil Science UnitFAO/IAEA Agriculture and Biotechnology LaboratorySeibersdorfAustria
  2. 2.Department of Land ManagementKatholieke Universiteit LeuvenHeverleeBelgium
  3. 3.Section of AgroecologyRoyal Veterinary and Agricultural UniversityTaastrupDenmark
  4. 4.Soil Science DepartmentIACR-Rothamsted, HarpendenHertfordshireUnited Kingdom
  5. 5.Soil Science UnitFAO/IAEA Agriculture and Biotechnology LaboratorySeibersdorfAustria

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