Plant and Soil

, Volume 221, Issue 1, pp 67–79 | Cite as

Modulation of amino acid metabolism in transformed tobacco plants deficient in Fd-GOGAT

  • S. Ferrario-Méry
  • A. Suzuki
  • C. Kunz
  • M.H. Valadier
  • Y. Roux
  • B. Hirel
  • C.H. Foyer
Article

Abstract

Tobacco (Nicotiana tabacum) plants expressing a

partial ferredoxin-dependent glutamine-2-oxoglutarate aminotransferase (Fd-GOGAT) cDNA in the antisense orientation under the control of the 35S promoter, were used to study the metabolism of amino acids, 2-oxoglutarate and ammonium following the transition from CO2 enrichment (where photorespiration is inhibited) to air (where photorespiration is a major process of ammonium production in leaves). The leaves of the lowest Fd-GOGAT expressors accumulated more foliar glutamine (Gln) and α-ketoglutarate (α-KG) than the untransformed controls in both growth conditions. Photorespiration-dependent increases in foliar ammonium, glutamine, α-KG and total amino acids were proportional to the decreases in foliar Fd-GOGAT activity. No change in endoprotease activity was observed following transfer to air in the Fd-GOGAT transformants or the untransformed controls which has similar activities over a broad range of pH values. We conclude that several pathways of amino acid biosynthesis are modified when NH3+ and Gln accumulate in leaves.

α-ketoglutarate amino acid ammonium assimilation glutamate synthase Nicotiana tabacum transgenic plant 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • S. Ferrario-Méry
  • A. Suzuki
  • C. Kunz
  • M.H. Valadier
  • Y. Roux
  • B. Hirel
  • C.H. Foyer

There are no affiliations available

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