Plant and Soil

, Volume 211, Issue 1, pp 59–67 | Cite as

Macropore sheath: quantification of plant root and soil macropore association

  • J.B. Stewart
  • C.J. Moran
  • J.T. Wood


Plants require roots to supply water, nutrients and oxygen for growth. The spatial distribution of roots in relation to the macropore structure of the soil in which they are growing influences how effective they are at accessing these resources. A method for quantifying root-macropore associations from horizontal soil sections is illustrated using two black vertisols from the Darling Downs, Queensland, Australia. Two-dimensional digital images were obtained of the macropore structure and root distribution for an area 55 × 55 mm at a resolution of 64 μm. The spatial distribution of roots was quantified over a range of distances using the K-function. In all specimens, roots were shown to be clustered at short distances (1–10 mm) becoming more random at longer distances. Root location in relation to macropores was estimated using the function describing the distance of each root to the nearest macropore. From this function, a summary variable, termed the macropore sheath, was defined. The macropore sheath is the distance from macropores within which 80% of roots are located. Measured root locations were compared to random simulations of root distribution to establish if there was a preferential association between roots and macropores. More roots were found in and around macropores than expected at random.

distance transformation macropore sheath root-soil interaction spatial statistics 


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  1. Baldwin J P, Tinker P B and Nye P H 1972Uptake of solutes by multiple root systems from soil. II. The theoretical effects of rooting density and pattern on uptake of nutrients from soil. Plant Soil 36, 693–708.CrossRefGoogle Scholar
  2. Bengough A G, Mackenzie C J and Diggle A J 1992Relations between root lengths densities and root intersections with horizontal and vertical planes using root growth modelling in 3-dimensions. Plant Soil 145, 245–252.CrossRefGoogle Scholar
  3. Borgefors G 1986Distance transformations in digital images. Computer Vision, Graphics, and Image Processing 34, 344–371.CrossRefGoogle Scholar
  4. Chadoeuf J, Kretzschmar A, Goulard M and Smettem K R J 1994 Description of the spatial interaction between earthworm burrows and cracks at the soil surface. In Soil Micromorphology: Studies in Management and Genesis. Eds A J Ringrose-Voase and G S Humphreys. Proc. IX Int. Working Meeting on Soil Micromorphology, Townsville, Australia, July, 1992. Dev. Soil Sci. 22, 521–530.Google Scholar
  5. Escamilla J A, Comerford N B and Neary D G 1991Spatial pattern of slash pine roots and its effect on nutrient uptake. Soil Sci. Soc. Am. J. 55, 1716–1722.CrossRefGoogle Scholar
  6. Hatano R and Sakuma T 1990The role of macropores on rooting pattern and movement of water and solutes in various field soils. Trans. of the 14th Congress of Int. Soc. Soil Sci. (Kyoto) II, 130–135.Google Scholar
  7. Hatano R, Iwanaga K, Okajima H and Sakuma T 1988Relationship between the distribution of soil macropores and root elongation. Soil Sci. Plant Nutr. 34, 535–546.Google Scholar
  8. Isbell R F 1996The Australian Soil Classification. Australian Soil and Land Survey Handbook Series, Vol. 4. CSIRO Australia. 143 p.Google Scholar
  9. Kooistra M J, Schoonderbeek D, Boone F R, Veen B W and Van Noordwijk M 1992Root-soil contact of maize, as measured by a thin-section technique. II. Effects of soil compaction. Plant Soil 139, 119–129.CrossRefGoogle Scholar
  10. Krebs M 1995Biogene Bodengefüge: Pflanzenartspezifische Oberboden-Mikrogefüge und Aspekte ihrer Entstehung. Hohenheimer Bodenkundliche Hefte 26, Universität Hohenheim, Stuttgart. 266 p.Google Scholar
  11. Meyer F 1978Contrast feature extraction. In Quantitative Analysis of Microstructures in Material Sciences, Biology and Medicine. Ed. J L Chermant. Special Issue of Practical Metallography 8, 374–380.Google Scholar
  12. Moran C J, Koppi A J, Murphy B W and McBratney A B 1988Comparison of the macropore structure of a sandy loam surface soil horizon subjected to two tillage treatments. Soil Use Manage. 4, 96–102.Google Scholar
  13. Moran C J, McBratney A B, Ringrose-Voase A J and Chartres C J 1989A method for the dehydration and impregnation of clay soil. J. Soil Sci. 40, 569–575.CrossRefGoogle Scholar
  14. Passioura J B 1991Soil structure and plant growth. Aust. J. Soil Res. 29, 717–728.CrossRefGoogle Scholar
  15. Pierret A, Moran C J and Pankhurst C E 1999Differentiation of soil properties related to the spatial association of wheat roots and soil macropores. Plant Soil 211, 51–58.CrossRefGoogle Scholar
  16. Rees K C J van, Hoskins J A and Hoskins W D 1994Analyzing root competition with dirichlet tessellation for wheat on three landscape positions. Soil Sci. Soc. Am. J. 58, 423–432.CrossRefGoogle Scholar
  17. Ripley B D 1977Modelling spatial patterns (with discussion). Journal of the Royal Statistical Society, Series B 39, 172–212.Google Scholar
  18. Rowlingson B S and Diggle P J 1993SPLANCS: Spatial point pattern analysis code in S-Plus. Computers & Geosciences 19, 627–655.CrossRefGoogle Scholar
  19. Tardieu F 1988Analysis of the spatial variability of maize root density. II. Distances between roots. Plant Soil 107, 267–272.CrossRefGoogle Scholar
  20. Tardieu F, Bruckler L and Lafolie F 1992Root clumping may affect the root water potential and the resistance to soil-root water transport. Plant Soil 140, 291–301.CrossRefGoogle Scholar
  21. Thompson C H and Beckmann G G 1959Soils and land use in the Toowoomba area, Darling Downs, Queensland. Soils and Land Use Series No. 28. Division of Soils, CSIRO, Melbourne. 78 p.Google Scholar
  22. Van Noordwijk M, Kooistra M J, Boone F R, Veen B W and Schoonderbeek D 1992Root-soil contact of maize, as measured by a thin-section technique. I. Validity of the method. Plant Soil 139, 109–118.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • J.B. Stewart
    • 1
  • C.J. Moran
  • J.T. Wood
  1. 1.Bureau of Rural SciencesKingstonAustralia

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