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Plant and Soil

, Volume 203, Issue 1, pp 37–46 | Cite as

Flax (Linum usitatissimum L.) depends on arbuscular mycorrhizal fungi for growth and P uptake at intermediate but not high soil P levels in the field

  • Ida Thingstrup
  • Gitte Rubaek
  • Erik Sibbesen
  • Iver Jakobsen
Article

Abstract

The contribution of indigenous arbuscular mycorrhizal fungi (AMF) to growth and phosphorus (P) uptake by oilseed flax (Linum usitatissimum L.) was examined in two field experiments covering soil P levels from 20–86 mg kg-1 NaHCO3-extractable P. The fumigant dazomet was applied to the soil in half of the plots to obtain control plants with reduced mycorrhiza formation. An extensive AMF colonization of up to 48% of the root length was established in untreated soil of both experiments, although P fertilization reduced colonization to 28–39% at the latest harvests. Fumigation markedly decreased or totally prevented AMF colonization throughout the experiments. Root growth responded to fumigation by increased total and specific root length. Shoot P uptake was decreased by fumigation at soil P levels lower than ca. 50 mg kg-1 whereas shoot growth was reduced by fumigation at soil P levels lower than ca. 40 mg kg-1. The effects of fumigation were ascribed to the suppression of mycorrhiza formation. The effect of the AMF increased with decreasing soil P levels. Phosphorus inflow through roots (based on shoot P uptake) was reduced more strongly by fumigation than total P uptake. The P inflow through fungal tissue in roots was estimated to 4 × 10-14 mol P cm-1 s-1. We conclude that AMF are essential to flax growth at soil P levels below ca. 40 mg P kg-1, which is representative of the conditions under which most flax is grown.

flax fumigation mycorrhiza P inflow P response curve root length density 

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References

  1. Anghinoni I and Barber S A 1980 Phosphorus influx and growth characteristics of corn roots as influenced by phosphorus supply. Agron. J. 72, 685–688.Google Scholar
  2. Anonymous 1995 Dyrkningsvejledning, Oliehør. Landskontoret for Planteavl, Århus.Google Scholar
  3. Barber S A 1984 Mechanisms of phosphorus uptake by plants and their application to plant selection for improved phosphorus up-take efficiency. In Third International Congress on Phosphorus Compounds, Brussels, Proceedings, pp 429–443. The World Phosphate Institute, Casablanca.Google Scholar
  4. Barea J M and Jeffries P 1995 Arbuscular mycorrhizas in sustainable soil-plant systems. In Mycorrhiza: Structure, Function, Molecular Biology and Biotechnology. Eds. A Varma and B Hock. pp 521–560. Springer, Berlin.Google Scholar
  5. Berta G, Trotta A, Fusconi A, Hooker J E, Munro M, Atkinson D, Giovannetti M, Morini S, Fortuna P, Tisserant B, Gianinazzi-Pearson V and Gianinazzi S 1995 Arbuscular mycorrhizal induced changes to plant growth and root system morphology in Prunus cerasifera. Tree Physiol. 15, 281–293.Google Scholar
  6. Bethlenfalvay G J and Linderman R G 1992 Mycorrhizae in sustainable agriculture. American Society of Agronomy, Madison. 124 p.Google Scholar
  7. Buwalda J G, Stribley D P and Tinker P B 1985 Effects of vesicular-arbuscular mycorrhizal infection in first second and third cereal crops. J. Agric. Sci. 105, 631–647.Google Scholar
  8. Graham J H and Eissenstat D M Field evidence for carbon cost of citrus mycorrhizas. New Phytol. (In press).Google Scholar
  9. Hayman D S 1975 The occurrence of mycorrhiza in crops as affected by soil fertility. In Endomycorrhizas. Eds. F E Sanders, B Mosse and P B Tinker. pp 495–509. Academic Press, London.Google Scholar
  10. Heckrath G, Brookes P C, Poulton P R and Goulding K W T 1995 Phosphorus leaching from soils containing different phosphorus concentrations in the Broadbalk experiment. J. Environ. Qual. 24, 904–910.Google Scholar
  11. Jakobsen I 1986 Vesicular-arbuscular mycorrhizas in field-grown crops III. Mycorrhizal infection and rates of phosphorus inflow in pea plants. New Phytol. 104, 573–581.Google Scholar
  12. Jakobsen I 1987 Effects of VA mycorrhiza on yield and harvest index of field grown pea. Plant Soil 98, 407–415.Google Scholar
  13. Jensen A and Jakobsen I 1980 The occurrence of vesicular-arbuscular mycorrhiza in barley and wheat grown in some Danish soils with different fertilizer treatments. Plant Soil 55, 403–414.Google Scholar
  14. Kronvang B, Græsbøll P, Larsen S E, Svendsen L M and Andersen H E 1996 Diffuse nutrient losses in Denmark. Water Sci. Technol. 33, 81–88.Google Scholar
  15. Lu S and Miller M H 1989 The role of VA mycorrhizae in the absorption of P and Zn by maize in field and growth chamber experiments. Can. J. Soil Sci. 69, 97–109.Google Scholar
  16. McGonigle T P, Evans D G, Miller M H 1990 Effect of degree of soil disturbance on mycorrhizal colonization and phosphorus absorption by maize in growth chamber and field experiments. New Phytol. 116, 629–636.Google Scholar
  17. Miller M H, McGonigle T P and Addy H D 1995 Functional ecology of vesicular arbuscular mycorrhizas as influenced by phosphate fertilization and tillage in an agricultural ecosystem. Crit. Rev. Biotechnol. 15, 241–255.Google Scholar
  18. Mol L 1995 Effect of plant roots on the germination of microsclerotia of Verticillium dahliae II. Quantitative analysis of the luring effect of crops. Eur.J. Plant Pathol. 101, 679–685.Google Scholar
  19. Murphy J and Riley I P 1962 A modified single solution method for the determination of phosphate in natural water. Anal. Chim. Acta 27, 31–36.Google Scholar
  20. Newman E I 1966 A method of estimating the total length of root in a sample. J. Appl. Ecol. 3, 139–145.Google Scholar
  21. Nye P H and Tinker P B 1977 Solute Movement in the Soil-root System. Blackwell, Oxford. 342 p.Google Scholar
  22. Olsen S R, Cole C V, Watanabe F S and Dean L A 1954 Estimation of available phosphorus in soils by extraction with sodium bicarbonate. Circular no. 939, USDA.Google Scholar
  23. Riley D and Barber S A 1971 Effect of ammonium and nitrate fertilization on phosphorus uptake as related to root-induced pH changes at the root-soil interface. Soil Sci. Soc. Am. Proc. 35, 301–306.Google Scholar
  24. Rovira A D 1976 Studies on soil fumigation I. Effects on ammonium, nitrate and phosphate in soil and on the growth, nutrition and yield of wheat. Soil Biol. Biochem. 8, 241–247.Google Scholar
  25. Sanders F E and Tinker P B 1973 Phosphate flow into mycorrhizal roots. Pestic. Sci. 4, 385–395.Google Scholar
  26. Sibbesen E and Sharpley A N 1997 Setting and justifying upper critical limits for phosphorus in soils. In Phosphorus Loss from Soil to Water. Eds. H Tunney, O T Carton, P C Brookes and A E Johnston. pp 151–176. CAB International Wallingford.Google Scholar
  27. Sibbesen E, Skjøth F and Christensen B T 1995 Soil and substance movement between plots in long-term field experiments. Danish Institute of Plant and Soil Science Report 29, 136–153.Google Scholar
  28. Thompson J P 1994 Inoculation with vesicular-arbuscular mycorrhizal fungi from cropped soil overcomes long-fallow disorder of linseed (Linum usitatissimum L.) by improving P and Zn uptake. Soil Biol. Biochem. 26, 1133–1143.Google Scholar
  29. Thompson J P 1996 Correction of dual phosphorus and zinc deficiency of linseed (Linum usitatissimum L.) with cultures of vesicular-arbuscular mycorrhizal fungi. Soil Biol. Biochem. 28, 941–951.Google Scholar
  30. Van der Zaag P, Fox R L, De la Pena R S and Yost R S 1979 P nutrition of casssava, including mycorrhizal effects on P, K, S, Zn and Ca uptake. Field Crops Res. 2, 253–263.Google Scholar
  31. Weber E, Saxena M C, George E and Marschner H 1993 Effect of vesicular-arbuscular mycorrhiza on vegetative growth and harvest index of chickpea grown in northern Syria. Field Crops Res. 32, 115–128.Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Ida Thingstrup
    • 1
  • Gitte Rubaek
    • 2
  • Erik Sibbesen
    • 2
  • Iver Jakobsen
    • 1
  1. 1.Plant Biology and Biogeochemistry Department, Risø National LaboratoryRoskildeDenmark
  2. 2.Department of Soil ScienceDanish Institute of Agricultural Sciences, Research Centre FoulumTjeleDenmark

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