QTL mapping for nodule number and common bacterial blight in Phaseolus vulgaris L.
- 111 Downloads
A recently developed bean RFLP linkage map was used to identify genetic elements affecting quantitative trait loci (QTLs) in two contrasting common bean genotypes, BAT-93 and Jalo EEP558, under two levels of mineral nitrogen: low – 0.25 mM NH4NO3 and a high – 6 mM NH4NO3. QTLs affecting nodule number (NN) and response to Xanthomonas campestris bv. phaseoli, which causes common bacterial blight (CBB) were identified and mapped. Analyses of 70 F2-derived F3 families, using the F1, the two parents, and a nodulation-defective mutant (Nod-) inoculated with R. tropici UM1899 under both levels of N showed significant differences (P#60;0.0001) among the F3 families for NN.
Under low N, three genomic regions influenced both traits, with seven linked markers. In three of the six regions influencing NN, higher NN was associated with the Jalo EEP-558 allele, whereas in only two regions was the BAT-93 allele associated with higher NN. One-way analysis of variance, with each marker as the independent variable and NN as the dependent variable, and interval mapping analysis identified four QTLs, which accounted for 45% of the total variation, and two additional QTLs near to yet unassigned loci. In linkage group D7, one QTL mapped to the same region as a QTL for CBB.
Under high N, three additional regions were linked to NN, one where the BAT-93 allele was closely associated with CH18 (chitinase), and the others where the Jalo EEP-558 allele was associated with CHS (chalcone synthetase) and PAL-1 (phenylalanine ammonia lyase). Four regions for CBB were mapped adjacent to or in the same region as a QTL for NN. Thus, N showed dual and opposite effects on the expression of NN and CBB. Analysis of these RFLP markers revealed these ‘hidden’ favorable alleles and can serve as an indirect selection tool to increase NN and resistance to CBB.
Unable to display preview. Download preview PDF.
- Bliss F A 1985 Breeding for enhancement of dinitrogen potential of common bean (Phaseolus vulgaris L.) In Nitrogen Fixation and CO2 Metabolism. Eds. P W Ludden and J E Burris. pp 303–310. Elsevier Publishers, NY.Google Scholar
- Bliss F A 1993 Breeding common bean for improved biological nitrogen fixation. Plant Soil 152, 71–79.Google Scholar
- Bliss F A, Pereira P A A, Araujo R S, Henson R A, Kmiecik K A, McFerson J R, Teixeira M G and da Silva C C 1989 Registration of five high nitrogen fixation common bean germplasm lines. Crop Sci. 29, 240–141.Google Scholar
- Centro Internacional De Agricultura Tropical (CIAT) 1983 Bean Program. Annual Report, Cali-Colombia.Google Scholar
- Davis J H C, Giller K E, Kipe-Nolt J and Awah M 1988 Non-nodulating mutants in common bean. Crop Sci. 28, 859–860.Google Scholar
- Gepts P, Nodari R, Tsai S M, Koinange E M K, Llaca V, Gilbertson R and Guzmán P 1993 Linkage mapping in common bean. Annu Rep. Bean Improv. Coop. 36, 24–38.Google Scholar
- Herridge D F and Danso S K A 1995 Enhancing crop legume N2 fixation through selection and breeding. Plant Soil 174, 51–82.Google Scholar
- Martínez-Romero E, Segovia L, Mercante F M, Franco A A, Graham P and Pardo MA 1991 Rhizobium tropici, a novel species nodulating Phaseolus vulgaris beans and Leucaena sp. trees. Int. J. Syst. Bact. 41, 417–426.Google Scholar
- McFerson J R 1983 Genetic and breeding studies of dinitrogen fixation in common bean (Phaseolus vulgaris L.). Ph.D. Thesis, University of Wisconsin, Madison.Google Scholar
- Mytton L R 1984 Developing a breeding strategy to exploit quantitative variation in symbiotic nitrogen fixation. Plant Soil 82, 329–335.Google Scholar
- Nodari R O, Koinange E M K, Kelly J D and Gepts P 1992 Towards an integrated linkage map of common bean. 1. Development of genomic DNA probes and levels of restriction fragment length polymorphism. Theor. Appl. Genet. 84, 186–192.Google Scholar
- Nodari R O, Tsai S M, Gilbertson R L and Gepts P 1993a Towards an integrated linkage map of common bean. 2. Development of an RFLP-based linkage map. Theor. Appl. Genetics 85, 513–520.Google Scholar
- Nutman P S 1984 Improving nitrogen fixation in legumes by plant breeding: the relevance of host selection experiments in red clover (Trifolium pratense L.) and subterraneum clover (T. subterraneum L.). Plant Soil 82, 285–301.Google Scholar
- Paterson A H, Lander E S, Hewitt J D, Peterson S, Lincoln S E, Lander E S, Tanksley S D 1991a Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335, 721–726.Google Scholar
- Peoples M B, Ladha J K and Herridge D F 1995 Enhancing legume N2 fixation through plant and soil management. Plant Soil 174, 83–101.Google Scholar
- Pereira P A A, Miranda B D, Attewell J R, Kmiecik K A and Bliss F A 1993 Selection for increased NN in common bean (Phaseolus vulgaris L.). Plant Soil 148, 203–209.Google Scholar
- Rosas J C and Bliss F A 1986 Host plant traits associated with estimates of nodulation and nitrogen fixation in common bean. Hort. Sci. 21, 287–289.Google Scholar
- SAS 1988. SAS/STAT User's Guide, Release 6.03 Edition. SAS Institute, Cary, NC, USA.Google Scholar
- Tsai S M, da Silva P M Cabezas W L and Bonetti R 1993 Minimizing the effect of mineral nitrogen on biological nitrogen fixation in common bean by increasing nutrient levels. Plant Soil 52, 131–138.Google Scholar
- Vincent J M 1970 A Manual for the Practical Study of Root Nodule Bacteria. Blackwell Scientific, Oxford.Google Scholar