Plant and Soil

, Volume 204, Issue 1, pp 135–145 | Cite as

QTL mapping for nodule number and common bacterial blight in Phaseolus vulgaris L.

  • S.M. Tsai
  • R.O. Nodari
  • D.H. Moon
  • L.E.A. Camargo
  • R. Vencovsky
  • P. Gepts
Article

Abstract

A recently developed bean RFLP linkage map was used to identify genetic elements affecting quantitative trait loci (QTLs) in two contrasting common bean genotypes, BAT-93 and Jalo EEP558, under two levels of mineral nitrogen: low – 0.25 mM NH4NO3 and a high – 6 mM NH4NO3. QTLs affecting nodule number (NN) and response to Xanthomonas campestris bv. phaseoli, which causes common bacterial blight (CBB) were identified and mapped. Analyses of 70 F2-derived F3 families, using the F1, the two parents, and a nodulation-defective mutant (Nod-) inoculated with R. tropici UM1899 under both levels of N showed significant differences (P#60;0.0001) among the F3 families for NN.

Under low N, three genomic regions influenced both traits, with seven linked markers. In three of the six regions influencing NN, higher NN was associated with the Jalo EEP-558 allele, whereas in only two regions was the BAT-93 allele associated with higher NN. One-way analysis of variance, with each marker as the independent variable and NN as the dependent variable, and interval mapping analysis identified four QTLs, which accounted for 45% of the total variation, and two additional QTLs near to yet unassigned loci. In linkage group D7, one QTL mapped to the same region as a QTL for CBB.

Under high N, three additional regions were linked to NN, one where the BAT-93 allele was closely associated with CH18 (chitinase), and the others where the Jalo EEP-558 allele was associated with CHS (chalcone synthetase) and PAL-1 (phenylalanine ammonia lyase). Four regions for CBB were mapped adjacent to or in the same region as a QTL for NN. Thus, N showed dual and opposite effects on the expression of NN and CBB. Analysis of these RFLP markers revealed these ‘hidden’ favorable alleles and can serve as an indirect selection tool to increase NN and resistance to CBB.

chalcone isomerase chalcone synthetase chitinase PAL phaseolin Phaseolus vulgaris QTL RFLP Rhizobium tropici Xanthomonas campestris bv. phaseoli 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bliss F A 1985 Breeding for enhancement of dinitrogen potential of common bean (Phaseolus vulgaris L.) In Nitrogen Fixation and CO2 Metabolism. Eds. P W Ludden and J E Burris. pp 303–310. Elsevier Publishers, NY.Google Scholar
  2. Bliss F A 1993 Breeding common bean for improved biological nitrogen fixation. Plant Soil 152, 71–79.Google Scholar
  3. Bliss F A, Pereira P A A, Araujo R S, Henson R A, Kmiecik K A, McFerson J R, Teixeira M G and da Silva C C 1989 Registration of five high nitrogen fixation common bean germplasm lines. Crop Sci. 29, 240–141.Google Scholar
  4. Centro Internacional De Agricultura Tropical (CIAT) 1983 Bean Program. Annual Report, Cali-Colombia.Google Scholar
  5. Coronado C, Zuanazzi J A S, Sallaud C, Quirion J-C, Esnault R, Jusson H-P, Kondorosi A and Ratet P 1995 Alfalfa root flavonoid production is nitrogen regulated. Plant Physiol. 108, 533–542.PubMedGoogle Scholar
  6. Davis J H C, Giller K E, Kipe-Nolt J and Awah M 1988 Non-nodulating mutants in common bean. Crop Sci. 28, 859–860.Google Scholar
  7. Gepts P, Nodari R, Tsai S M, Koinange E M K, Llaca V, Gilbertson R and Guzmán P 1993 Linkage mapping in common bean. Annu Rep. Bean Improv. Coop. 36, 24–38.Google Scholar
  8. Graham P H 1981 Some problems of nodulation and symbiotic nitrogen fixation in Phaseolus vulgaris L.: a review. Field Crops Res. 4, 93–112.CrossRefGoogle Scholar
  9. Herridge D F and Danso S K A 1995 Enhancing crop legume N2 fixation through selection and breeding. Plant Soil 174, 51–82.Google Scholar
  10. Lander E S and Botstein D 1989 Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121, 185–199.PubMedGoogle Scholar
  11. Lander E S, Green P, Abrahamson J, Barlow A, Daly M, Lincoln S E and Newburg L 1987 MAPMAKER: an interative computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174–181.PubMedGoogle Scholar
  12. Martínez-Romero E, Segovia L, Mercante F M, Franco A A, Graham P and Pardo MA 1991 Rhizobium tropici, a novel species nodulating Phaseolus vulgaris beans and Leucaena sp. trees. Int. J. Syst. Bact. 41, 417–426.Google Scholar
  13. McFerson J R 1983 Genetic and breeding studies of dinitrogen fixation in common bean (Phaseolus vulgaris L.). Ph.D. Thesis, University of Wisconsin, Madison.Google Scholar
  14. Mytton L R 1984 Developing a breeding strategy to exploit quantitative variation in symbiotic nitrogen fixation. Plant Soil 82, 329–335.Google Scholar
  15. Nodari R O, Koinange E M K, Kelly J D and Gepts P 1992 Towards an integrated linkage map of common bean. 1. Development of genomic DNA probes and levels of restriction fragment length polymorphism. Theor. Appl. Genet. 84, 186–192.Google Scholar
  16. Nodari R O, Tsai S M, Gilbertson R L and Gepts P 1993a Towards an integrated linkage map of common bean. 2. Development of an RFLP-based linkage map. Theor. Appl. Genetics 85, 513–520.Google Scholar
  17. Nodari R O, Tsai S M, Guzmán P and Gept P 1993b Towards an integrated linkage map of common bean. 3. Mapping genetic factors underlying Rhizobium nodule number, common bacterial blight, and seed weight. Genetics 134, 341–350.PubMedGoogle Scholar
  18. Nutman P S 1984 Improving nitrogen fixation in legumes by plant breeding: the relevance of host selection experiments in red clover (Trifolium pratense L.) and subterraneum clover (T. subterraneum L.). Plant Soil 82, 285–301.Google Scholar
  19. Paterson A H, Lander E S, Hewitt J D, Peterson S, Lincoln S E, Lander E S, Tanksley S D 1991a Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335, 721–726.Google Scholar
  20. Paterson A H, Lander E S, Hewitt J D, Peterson S, Lincoln S E, Lander E S, Tanksley S D 1991b Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, environments. Genetics 127, 181–197.PubMedGoogle Scholar
  21. Peoples M B, Ladha J K and Herridge D F 1995 Enhancing legume N2 fixation through plant and soil management. Plant Soil 174, 83–101.Google Scholar
  22. Pereira P A A, Miranda B D, Attewell J R, Kmiecik K A and Bliss F A 1993 Selection for increased NN in common bean (Phaseolus vulgaris L.). Plant Soil 148, 203–209.Google Scholar
  23. Rosas J C and Bliss F A 1986 Host plant traits associated with estimates of nodulation and nitrogen fixation in common bean. Hort. Sci. 21, 287–289.Google Scholar
  24. SAS 1988. SAS/STAT User's Guide, Release 6.03 Edition. SAS Institute, Cary, NC, USA.Google Scholar
  25. Tsai S M, da Silva P M Cabezas W L and Bonetti R 1993 Minimizing the effect of mineral nitrogen on biological nitrogen fixation in common bean by increasing nutrient levels. Plant Soil 52, 131–138.Google Scholar
  26. Vincent J M 1970 A Manual for the Practical Study of Root Nodule Bacteria. Blackwell Scientific, Oxford.Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • S.M. Tsai
    • 1
  • R.O. Nodari
    • 2
  • D.H. Moon
    • 1
  • L.E.A. Camargo
    • 3
  • R. Vencovsky
    • 3
  • P. Gepts
    • 4
  1. 1.Centro de Energia Nuclear na Agricultura/USPPiracicaba-S.PBrazil
  2. 2.UFSC/MEC-CaixaFlorianópolis-SCBrazil
  3. 3.ESALQ/USPPiracicaba-S.PBrazil
  4. 4.Dept. of Agronomy and Range ScienceUC-DavisUSA

Personalised recommendations