Plant and Soil

, Volume 189, Issue 2, pp 239–244 | Cite as

The natural 15N abundance of sugarcane and neighbouring plants in Brazil, the Philippines and Miyako (Japan)

  • T. Yoneyama
  • T. Muraoka
  • T.H. Kim
  • E.V. Dacanay
  • Y. Nakanishi


The contribution of nitrogen fixation by sugarcane plants grown in the fields at various sites in Brazil, at a few sites in the Philippines and in Miyako Island (Japan) was examined using the difference in natural 15N abundance (δ15N) in the leaves of sugarcane compared to those of neighbouring plants. The sugarcane plants from Três Poutas of Minas Gerais State, Cerquilho, Novo Horizonte, Igarapava, Itápolis, Assis of São Paulo State in Brazil showed lower δ15N values than the neighbouring plants with an average contribution of N2 fixation to total plant N of 30% (ranging from zero to 72%). The sugarcane from Gerona in the Philippines and from three sites in Miyako Island indicated significant contribution of N2 fixation. In other sites examined in Brazil and in the Philippines, the contribution of N2 fixation was not clear.

Brazil endophyte Miyako (Japan) natural 15N abundance (δ15N) N2 fixation the Philippines sugarcane 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Boddey R M, Reis VM, Urquiaga S, da Silva L G, Reis F B, Baldani J I and Döbereiner J 1995 N2 fixation in sugarcane: The role of Acetobacter diazotrophicus. InNitrogen Fixation: Fundamentals and Applications. Eds. I A Tikhonovich et al. pp 641-646. Kluwer Academic Publishers, Dordrecht.Google Scholar
  2. Boddey R M and Victoria R L 1986 Estimation of biological nitrogen fixation associated with Brachiariaand Paspalumgrasses using 15N labellled organicmatter and fertilizer. Plant Soil 90, 265-292.Google Scholar
  3. Cavalcante V A and Döbereiner J 1988 A new acid-tolerant nitrogen-fixing bacterium associated with sugarcane. Plant Soil 108, 23- 51.Google Scholar
  4. Döbereiner J 1961 Nitrogen-fixing bacteria of the genus BeijerinchiaDerx in the rhizosphere of sugarcane. Plant Soil 15, 211-217.Google Scholar
  5. Döbereiner J, Day J M and Dart P J 1972 Nitrogenase activity in the rhizosphere of sugarcane and some other tropical grasses. Plant Soil 37, 191-196.Google Scholar
  6. Dong Z, Canny M J, McCully M E, Roboredo M R, Cabadillo C F, Ortega E and Rodés R 1994 A nitrogen-fixing endophyte of sugarcane stems. A new role for the apoplast. Plant Physiol. 105, 1139-1147.Google Scholar
  7. Hoering T and Ford H T 1960 The isotope effect in the fixation of nitrogen by Azotobacter. J. Am. Chem. Soc. 82, 376-378.Google Scholar
  8. James E K, Reis V M, Olivares F L, Baldami J I and Döbereiner J 1994 Infection of sugarcane by the nitrogen-fixing bacterium Acetobacter diazotrophicus. J. Exp. Bot. 45, 757-766.Google Scholar
  9. Lee K K, Wani S P, Yoneyama T, Trimurtula N and Harikrishnan R 1994 Associative N2-fixation in pearlmillet and sorghum: Levels and response to inoculation. Soil Sci. Plant Nutr. 40, 477-484.Google Scholar
  10. Lima E, Boddey R M and Döbereiner J 1987 Quantification of biological nitrogen fixation associated with sugarcane using a 15-N aided nitrogen balance. Soil Biol. Biochem. 19, 167-170.Google Scholar
  11. Malik K A, Bilal R, Rasul G, Mahmood K and Sajjad M I 1991 Associative N2-fixation in plants growing in saline sodic soils and its relative quantification based on 15N natural abundance. Plant Soil 137, 67-74.Google Scholar
  12. Ruschel A P 1975 Nitrogen-15 tracing of N-fixation with soil grown sugarcane seedling. Soil Biol. Biochem. 7, 181-182.Google Scholar
  13. Shearer G and Kohl D H 1986 N2-fixation in fields seedlings: Estimations based on natural 15N abundance. Aust. J. Plant Physiol. 13, 699-756.Google Scholar
  14. Urquiaga S, Cruz K H S and Boddey R M 1992 Contribution of nitrogen fixation to sugarcane: nitrogen-15 and nitrogen-balance estimates. Soil Sci. Soc. Am. J. 56, 105-114.Google Scholar
  15. Watanabe I, Yoneyama T, Padre B and Ladha J K 1987 Difference in natural abundance of 15N in several rice (Oryza sativaL.) varieties: Appplication for evaluating N2 fixation. Soil Sci. Plant Nutr. 33, 407-415.Google Scholar
  16. Yoneyama T, Muraoka T, Murakami T and Boonkerd N 1993 Natural abundance of 15N in tropical plants with emphasis on tree legumes. Plant Soil 53, 295-304.Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • T. Yoneyama
    • 1
  • T. Muraoka
    • 2
  • T.H. Kim
    • 3
  • E.V. Dacanay
    • 4
  • Y. Nakanishi
    • 5
  1. 1.Plant Nutrition Diagnosis Laboratory, National Agriculture Research CenterTsukuba, IbarakiJapan
  2. 2.Centro de Energia Nuclear na Agricultura (CENA)Universidade de São Paulo, PiracicabaSão PauloBrazil
  3. 3.Institute of Development for Livestock ProductionGyeongsang National UniversityChinju, KyungnamKorea
  4. 4.Bureau of Soils and Water Management, DilimanQuezon cityPhilippines
  5. 5.Miyako Subtropical Experimental FarmTokyo University of AgricultureGusukube, Miyako, OkinawaJapan

Personalised recommendations