Plant and Soil

, Volume 195, Issue 2, pp 221–232 | Cite as

Estimating respiration of roots in soil: Interactions with soil CO2, soil temperature and soil water content

  • Tjeerd J. Bouma
  • Kai L. Nielsen
  • David M. Eissenstat
  • Jonathan P. Lynch

Abstract

Little information is available on the variability of the dynamics of the actual and observed root respiration rate in relation to abiotic factors. In this study, we describe I) interactions between soil CO2 concentration, temperature, soil water content and root respiration, and II) the effect of short-term fluctuations of these three environmental factors on the relation between actual and observed root respiration rates. We designed an automated, open, gas-exchange system that allows continuous measurements on 12 chambers with intact roots in soil. By using three distinct chamber designs with each a different path for the air flow, we were able to measure root respiration over a 50-fold range of soil CO2 concentrations (400 to 25000 ppm) and to separate the effect of irrigation on observed vs. actual root respiration rate. All respiration measurements were made on one-year-old citrus seedlings in sterilized sandy soil with minimal organic material.

Root respiration was strongly affected by diurnal fluctuations in temperature (Q10 = 2), which agrees well with the literature. In contrast to earlier findings for Douglas-fir (Qi et al., 1994), root respiration rates of citrus were not affected by soil CO2 concentrations (400 to 25000 ppm CO2; pH around 6). Soil CO2 was strongly affected by soil water content but not by respiration measurements, unless the air flow for root respiration measurements was directed through the soil. The latter method of measuring root respiration reduced soil CO2 concentration to that of incoming air. Irrigation caused a temporary reduction in CO2 diffusion, decreasing the observed respiration rates obtained by techniques that depended on diffusion. This apparent drop in respiration rate did not occur if the air flow was directed through the soil. Our dynamic data are used to indicate the optimal method of measuring root respiration in soil, in relation to the objectives and limitations of the experimental conditions.

citrus Citrus volkameriana Tan. & Pasq. CO2-diffusion gradient root respiration soil CO2 concentration Volkamer lemon 

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References

  1. Amthor J S 1991 Respiration in a future, higher-CO2 world. Plant Cell. Environ. 14, 13–20.Google Scholar
  2. Bloom A J, Sukrapanna S S and Warner R L 1992 Root respiration associated with ammonium and nitrate absorption and assimilation by barley. Plant Physiol. 94, 85–90.Google Scholar
  3. Chapman S B 1979 Some interrelationships between soil and root respiration in lowland Calluna heathland in southern England. J. Ecol. 67, 1–20.Google Scholar
  4. Duenas C, Fernandez M C, Carretero J, Liger E and Perez M 1995 Emission of CO2 from some soils. Chemosphere 30, 1875–1889.Google Scholar
  5. Edwards N T 1982 The use of soda-lime for measuring root respiration rates in terrestical systems. Pedobiologia 23, 321–330.Google Scholar
  6. Edwards N T 1991 Root and soil respiration responses to ozone in Pinus taede L. seedlings. New Phytol. 118, 315–321.Google Scholar
  7. Frossard J S 1985 Effect de la temperature des racines sur leur respiration et sur la croissance de plantules de deux hybrides de mais. Agronomie 5, 719–725.Google Scholar
  8. Garcia R L, Idso S B, Wall G W and Kimball B A 1994 Changes in net photosynthesis and growth of Pinus eldarica seedlings in response to atmospheric CO2 enrichment. Plant Cell Environ. 17, 971–978.Google Scholar
  9. Hoagland D R and Arnon D I 1939 The water-culture method for growing plants without soil. University of California, Agricultural Experimental Station Circular 347, Berkeley, CA.Google Scholar
  10. Idso S B and Kimball B A 1992a Effects of atmospheric enrichment on photosynthesis, respiration and growth of sour orange trees. Plant Physiol. 99, 341–343.Google Scholar
  11. Idso S B and Kimball B A 1992b Seasonal fine root biomass development of sour orange trees grown in atmospheres of ambient and elevated CO2 concentration. Plant Cell Environ. 15, 337–341.Google Scholar
  12. Johnson D, Geisinger D, Walker R, Newman J, Vose J, Elliot K and Ball T 1994 Soil pCO2, soil respiration, and root activity in CO2-fumigated and nitrogen-fertilized ponderosa pine. Plant Soil 165, 129–138.Google Scholar
  13. Kanemasu E T, Powers W L and Sij J W 1974 Field chamber measurements of CO2 flux from soil surface. Soil Science 118, 233– 237.Google Scholar
  14. Kosola K R and Eissenstat D M 1994 The fate of surface roots of citrus seedlings in dry soils. J. Exp. Bot. 45, 1639–1645.Google Scholar
  15. Lambers H, Scheurwater I and Atkin O K 1996 Respiratory patterns in roots in relation to their functioning. In Plant Roots, the Hidden Half. Eds. Y Waisel, A Eshel and K Kafkaki. pp 323–362. Marcel Dekker, Inc., New York.Google Scholar
  16. Layzell D B, Hunt S, King B J, Walsh K B and Weagle G E 1989 A multichannel system for steady-state and continuous measurements of gas exchange from legume roots and nodules. In Application of continuous and steady-state methods to root biology. Eds. J G Torrey and L J Winship. pp 1–28. Kluwer Academic Publisher, Dordrecht.Google Scholar
  17. Lawrence W T and Oechel WC 1983 Effects of soil temperature on the carbon exchange of taiga seedlings. I. Root respiration. Can. J. For. Res. 13, 840–849.Google Scholar
  18. Nobel P S and Palta J A 1989 Soil 02 and CO2 effects on root respiration of cacti. Plant Soil 120, 263–271.Google Scholar
  19. Palta J A and Nobel P S 1989a Influence of water status, temperature, and root age on daily patterns of root respiration for two cactus species. Ann. Bot. 63, 651–662.Google Scholar
  20. Palta J A and Nobel P S 1989b Influence of soil O2 and CO2 on root respiration for Agave deserti. Physiol. Plant. 76, 187–192.Google Scholar
  21. Poorter H, Gifford R M, Kriedemann P E and Wong S C 1992 A quantitative analysis of dark respiration and carbon content as factors in the growth response of plants to elevated CO2. Aust. J. Bot. 40, 501–513.Google Scholar
  22. Qi J, Marshall J D and Mattson K G 1994 High soil carbon dioxide concentrations inhibit root respiration of Douglas fir. New Phytol. 128, 435–442.Google Scholar
  23. Reuveni J and Gale J 1985 The effect of high levels of carbon dioxide on dark respiration and growth of plants. Plant Cell Environ. 8, 623–628.Google Scholar
  24. Rochette P, Desjardins R L and Pattey E 1991 Spatial and temporal variability of soil respiration in agricultural fields. Can. J. Soil Sci. 71, 189–196.Google Scholar
  25. Rogers H H and Runion G B 1994 Plant responses to atmospheric CO2 enrichment with emphasis on roots and the rhizosphere. Environ. Pollution 83, 155–189.Google Scholar
  26. Rohlf F J and Sokal R R 1981 Statistical Tables, second edition. W.H. Freeman and Company, New York.Google Scholar
  27. Smakman G and Hofstra R J J 1982 Energy metabolism of Plantago lanceolata, as affected by change in root temperature. Physiol. Plant. 56, 33–37.Google Scholar
  28. Solomos T 1987 Principles of gas exchange in bulky plant tissues. Hort. Science 22, 766–771.Google Scholar
  29. Sowell J B and Spomer G G 1986 Ecotypic variation in root respiration rate along elevational populations of Abies lasiocarpa and Picea engelmannii. Oecologia 68, 375–379.Google Scholar
  30. Topp G C 1993 Soil water content. In Soil Sampling and Methods of Analysis. Ed. M R Carter. pp 541–557. Can. Soc. Soil Sci., Lewis Publishers.Google Scholar
  31. Topp G C and Davis J L 1985 Measurement of soil water content using time-domain reflectometry (TDR): a field evaluation. Soil Sci. Soc. Am. J. 49, 19–24.Google Scholar
  32. Veen B W 1980 Energy cost of ion transport. In Genetic Engineering of Osmoregulation. Impact on Plant Productivity for Food, Chemicals and Energy. Eds. D W Rains, R C Valentine and C Holaender. pp 187–195. Plentum Press, New York.Google Scholar
  33. Weger H G and Guy R D 1991 Cytochrome and alternative pathway respiration inwhite spruce (Picea glauca) roots.Effects of growth and measurement temperature. Physiol. Plant. 83, 675–681.Google Scholar
  34. Wullschleger S D, Norby R J and Gunderson C A 1992 Growth and maintenance respiration in leaves of Liriodendron tulipifera L. exposed to long term carbon dioxide enrichment in the field. New Phytol. 121, 515–523.Google Scholar
  35. Wullschleger S D, Ziska L H and Bunce J A 1994 Respiratory response of higher plants to atmospheric CO2 enrichment. Physiol. Plant. 90, 221–229.Google Scholar
  36. Zimmerman R C, Smith R D and Alberte R S 1989 Thermal acclimation and whole-plant carbon balance in Zostera marina L. (eelgrass). J. Exp. Mar. Biol. Ecol. 130, 93–109.Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Tjeerd J. Bouma
  • Kai L. Nielsen
  • David M. Eissenstat
  • Jonathan P. Lynch

There are no affiliations available

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