Plant and Soil

, Volume 197, Issue 1, pp 19–23 | Cite as

Isolation and identification of N2-fixing microorganisms from the rhizosphere of Capparis spinosa (L.)

  • Galdino Andrade
  • Eduardo Esteban
  • Leonardo Velasco
  • María J. Lorite
  • Eulogio J. Bedmar


Four bacterial strains, Pseudomonas stutzeri var. mendocina, Comamonas sp., Agrobacterium tumefaciens biovar. 2 and Sphingobacterium sp., isolated from the rhizosphere of wild-grown caper (Capparis spinosa L.) plants were able to fix N2 as shown by their growth in nitrogen-free medium and by the acetylene reduction test. P. stutzeri var. mendocina and Comamonas sp. contained DNA homologous to the Klebsiella pneumoniae M5a1 nifHDK genes. No hybridization was found with total DNA from either A. tumefaciens biovar. 2 or Sphingobacterium sp. using nifHDK probes from either K. pneumoniae or Rhizobium meliloti.

Agrobacterium Capparis spinosa Comamonas N2 fixation Pseudomonas rhizosphere Sphingobacterium 


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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Galdino Andrade
    • 1
  • Eduardo Esteban
    • 2
  • Leonardo Velasco
    • 1
  • María J. Lorite
    • 1
  • Eulogio J. Bedmar
    • 1
  1. 1.Departamento de Microbiología del Suelo y Sistemas SimbióticosEstación Experimental del ZaidínGranadaSpain
  2. 2.Departamento de Agroecología y Protección VegetalEstación Experimental del ZaidínGranadaSpain

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