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Hydrobiologia

, Volume 431, Issue 2–3, pp 155–163 | Cite as

Gut content analysis of Gammarus lacustris from a Siberian lake using biochemical and biophysical methods

  • Michail I. Gladyshev
  • Anna Y. Emelianova
  • Galina S. Kalachova
  • Tatiana A. Zotina
  • Nikolai A. Gaevsky
  • Michail D. Zhilenkov
Article

Abstract

We studied the gut contents of Gammarus lacustris from the littoral of Shira lake, a Siberian salt lake, by microscopic analysis in conjunction with measuring of fluorescence of chlorophyll and composition of fatty acids. Gammarus ingested primarily fresh seston, probably settled on the bottom. Additionally, the amphipods obtained essential polyunsaturated fatty acids of ω3 family by selectively consuming bottom sediment particles. A considerable part of the ingested seston comprised cells of Botryococcus sp., which passed the intestinal tract in living form and their photosynthetic activity increased. This viable gut passage was likely one of the causes of bloom of Botryococcusin the littoral. Unlike a number of literature data, there was no selective digestion of any fatty acid species, including polyunsaturated acids, in the intestinal tract.

fatty acid composition viable gut passage chlorophyll fluorescence 

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References

  1. Bligh, E. G. &; W. J. Dyer, 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. 37: 911–917.Google Scholar
  2. Campbell, R. C., 1967. Statistics for Biologists. University Press, Cambridge: 242 pp.Google Scholar
  3. Canuel, E. A., J. E. Cloern, D. B. Ringelberg &; J. B. Guckert, 1995. Molecular and isotopic tracers used to examine sources of organic matter and its incorporation into food webs of San Francisco Bay. Limnol. Oceanogr. 40: 67–81.Google Scholar
  4. Clarke, A., L. J. Holmes, &; C. C. E. Hopkins, 1987. Lipids in Arctic food chain: Calanus, Bolinopsis, Beroe. Sarsia 72: 41–48.Google Scholar
  5. Clarke, A., A. Skadsheim &; L. J. Holmes, 1985. Lipid biochemistry and reproductive biology in two species of Gammaridae (Crustacea: Amphipoda). Mar. Biol. 88: 247–263.Google Scholar
  6. Claustre, H., J. C. Marty, L. Cassiani &; J. Dagaut, 1988/89. Fatty acid dynamics in phytoplankton and microzooplankton communnities during a spring bloom in the coastal Ligurian Sea: ecological implications. Mar. Microbial. Food Webs 3: 51–66.Google Scholar
  7. Claustre, H., S. A. Poulet, R. Williams, F. Ben-Mlih, V. Martin-Jezequel &; J. C. Marty, 1992. Relationship between the qualitative nature of particles and copepod faeces in the Irish Sea. Mar. Chem. 40: 231–248.Google Scholar
  8. Ederington, M., G. B. McManus &; R. Harvey, 1995. Trophic transfer of fatty acids, sterols and a triterpenoid alcohol between bacteria, a ciliate, and the copepod Acartia tonsa. Limnol. Oceanogr. 40: 860–867.Google Scholar
  9. Gayte, X. &; D. Fontvieille, 1997. Autochthonous vs. allochthonous organic matter ingested by a macroinvertebrate in headwater streams: Gammarus sp. as a biological probe. Arch. Hydrobiol. 140: 23–36.Google Scholar
  10. Goulden, C. E. &; A. R. Place, 1990. Fatty acid synthesis and accumulation rates in daphnids. J. Exp. Zool. 256: 168–178.Google Scholar
  11. Jefers J., 1981. An introduction to system analysis: with ecological application. 'Mir', Moscow: 252 pp. (translated from English).Google Scholar
  12. Keller, A. A., 1987. Mesocosm studies of DCMU-enhanced fluorescence as a measure of phytoplankton photosynthesis. Mar. Biol. 96: 107–114.Google Scholar
  13. Knisley, K. &; W. Geller, 1986. Selective feeding of four zooplankton species on natural lake phytoplankton. Oekologia. 69: 86–94.Google Scholar
  14. Kuzmin, G. V., 1975. Species composition and abundance of phytoplankton. In Mordukhai-Boltovskoi, F. D. (ed.), Methods for Investigation of Inland Aquatic Biogeocenoses. Nauka, Moscow: 73–87 (in Russian).Google Scholar
  15. Muller-Navarra, D., 1995. Evidence that a highly unsaturated fatty acid limits Daphnia growth in nature. Arch. Hydrobiol. 132: 297–307.Google Scholar
  16. Napolitano, G. E. &; R. G. Ackman, 1989. Lipids and hydrocarbons in Corophium volutator from Minas Basin, Nova Scotia. Mar. Biol. 100: 333–338.Google Scholar
  17. Nichols, P. D., J. K. Volkman &; D. A. Everitt, 1989. Occurrence of cis-6-hexadecenoic acid and other unusual monounsaturated fatty acids in the lipids of oceanic particulate matter. Ocean. Acta. 12: 393–403.Google Scholar
  18. Porter, K. G., 1976. Enhancement of algal growth and productivity by grazing zooplankton. Science 192: 1332–1336.Google Scholar
  19. Quigley, M. A. &; H. A. Vanderploeg, 1991. Ingestion of live filamentous diatoms by the Great Lakes amphipod Diporea sp.: A case study of the limited value of gut contents analysis. Hydrobiologia 223 (Dev. Hydrobiol. 30): 141–148.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Michail I. Gladyshev
  • Anna Y. Emelianova
  • Galina S. Kalachova
  • Tatiana A. Zotina
  • Nikolai A. Gaevsky
  • Michail D. Zhilenkov

There are no affiliations available

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