Euphytica

, Volume 110, Issue 1, pp 21–34 | Cite as

Study of genetic diversity in Indian and exotic sesame (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers

  • K. Venkataramana Bhat
  • Prashant P. Babrekar
  • Suman Lakhanpaul
Article

Abstract

Fifty-eight accessions of sesame (Sesamum indicum L.), an important oil seed crop of the tropics and subtropics were analysed using random amplified polymorphic DNA (RAPD) technique. The material analysed comprised 36 collections from 18 different states of India and four adjoining countries of the Indian subcontinent, and 22 exotic accessions from 21 sesame growing countries around the world. The results from PCR amplifications with the selected 24 random 10-mer primers were statistically analysed. The value of Jaccard’s similarity coefficients ranged from 0.19 to 0.89. The results indicated the presence of high level of genetic diversity. However, the extent of genetic diversity was greater in the collections from Indian subcontinent as compared to the exotics. Among the Indian accessions, the collections from Rajasthan and North-eastern states were highly diverse. The phenetic analysis grouped 48 out of 58 accessions in six clusters and the remaining highly diverse accessions were placed outside these close-knit clusters. The Bootstrap estimates obtained by Wagner parsimony analysis were significant for seven out of 49 nodes in the majority-rule consensus tree (<95% occurrence). The results of both the analyses were, however, broadly comparable when the constitution of the individual clusters were considered. The principal components analysis indicated that the first two components accounted for only 21% of the total variations and in order to explain <75% of variations 18 components were required. The high level of genetic diversity prevalent among the Indian collections is probably indicative of the nativity of this crop species. Similarly, the relatively lower level of polymorphism in exotic germplasm could be ascribed to the comparatively recent introductions of limited germplasm of this crop into some of the non-traditional sesame growing countries.

genetic diversity germplasm molecular markers RAPD sesame 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ashri, A., 1988. Sesame breeding-objectives and approaches. In: Oil Crops-Sunflower, Linseed and Sesame. Proc. 4th Oil Crop Network Workshop, Njoro, Kenya, January 1988. IDRCMR20e, IDRC.Google Scholar
  2. Ashri, A., 1989. Sesame. In: G. Roebblen, R.K. Downey & A. Ashri (Eds), Oil Crops of the World, pp. 375-387. McGraw Hill Publishing Co., New York.Google Scholar
  3. Ashri, A., 1995. Sesame research overview: current status, perspectives and priorities. In: M.R. Bennet & I.M. Wood (Eds), Proceedings of the First Australian Sesame Workshop. Darwin and Katherine, Northern Territory.Google Scholar
  4. Bedigian, D., 1981. Origin, diversity, exploration and collection of sesame. Sesame: Status and Improvement. Proceedings of Expert Consultation, Rome, Italy. 8-12 December, 1980. FAO Plant Production and Protection Paper 29, pp. 164-169.Google Scholar
  5. Bedigian, D., 1984. Sesamum indicum L.: Crop Origin, Diversity, Chemistry and Ethnobotany, Ph D. Dissertation, University of Illinois, Urbana-Champaign.Google Scholar
  6. Bedigian, D. & J.R. Harlan, 1986. Evidence for the cultivation of sesame in the ancient world. Econ Bot 40: 137-154.Google Scholar
  7. Bisht, I.S., R.K. Mahajan, T.R. Loknathan & R.C. Agarwal, 1998. Diversity in Indian sesame collection and stratification of germplasm accessions in different diversity groups. Genetic Resources and Crop Evolution 45: 325-335.CrossRefGoogle Scholar
  8. Bhat, K.V. & R.L. Jarret, 1995. Random amplified polymorphic DNA and genetic diversity in Indian Musa germplasm. Genetic Resources and Crop Evolution 42: 107-118.Google Scholar
  9. Brar, G.S. & K.L. Ahuja, 1979. Sesame, its culture, genetics, breeding and biochemistry. Ann Rev Pl Sci 1: 245-313. Kalyani Publ., New Delhi, India.Google Scholar
  10. Brunk, C.F., K.C. Jones & T.W. James, 1979. Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem 92: 497-500.PubMedCrossRefGoogle Scholar
  11. FAO, 1993. Production Yearbook 1992, Food and Agricultural Organisation, Rome 46: 231-232.Google Scholar
  12. FAO, 1994. Trade Year Book 1993, Food and Agricultural Organisation, Rome 47: 113.Google Scholar
  13. Felsenstein, J. 1988. Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 22: 521-565.PubMedCrossRefGoogle Scholar
  14. Felsenstein, J., 1989. PHYLIP-Phylogenetic Inference package (Ver. 3.2). Cladistics 5: 164-166.Google Scholar
  15. Hadrys, H., M. Balick & B. Schierwater, 1992. Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Molecular Ecology 1: 55-63.PubMedGoogle Scholar
  16. Hamrick, J.L. & M.J.W. Godt, 1989. Allozyme diversity in plants. In: A.H.D. Brown, M.T. Clegg, A.L. Kahler & B.S. Wier (Eds), Plant Population, Genetics, Breeding and Genetic Resources, pp. 318-370. Sinaur Associates, Sunderland. MA, USA.Google Scholar
  17. Isshiki, S. & T. Umezaki, 1997. Genetic variations of isozymes in cultivated sesame. Euphytica 93: 375-377.CrossRefGoogle Scholar
  18. Jaccard, P., 1908. Nouvelles recherchés sur la distribution florale. Bull Soc Vaud Sci Nat 44: 223-270.Google Scholar
  19. Joshi, A.B., 1961. Sesamum. Indian Central Oilseed Committee, Hyderabad, India.Google Scholar
  20. Mazanni, B., 1983. Ajonjoli, In: Cultivo y Majoramiento de Plantas Oleaginosas. FONAIP. Caracas, Venezuela. pp. 162-224.Google Scholar
  21. Monte, J.V., C.L. McIntyre & J.P. Gustafson, 1993. Analysis of phylogenetic relationships in the Triticeae tribe using RFLPs. Theor Appl Genet 86: 649-655.CrossRefGoogle Scholar
  22. Rana, R.S., R.K. Arora, T.R. Loknathan & D.P. Patel., 1994. Sesame genetic resources in India: their diversity, utilisation and conservation. In: R.K. Arora & K.W. Riley (Eds), Sesame Biodiversity in Asia-Conservation, Evaluation and Improvement. pp 109-134, International Plant Genetic Resources Institute, Office for South Asia, New Delhi.Google Scholar
  23. Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgenson & A.W. Allard, 1984. Ribosomal spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci (USA) 81: 8014-8018.CrossRefGoogle Scholar
  24. Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning — A Laboratory Manual. 2nd Edition. Cold Spring Harbor, NY, USA.Google Scholar
  25. Song, K.M., T.C. Osborn & P.H. Williams, 1988. Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). Theor Appl Genet 75: 784-794.CrossRefGoogle Scholar
  26. Thangavelu, S., 1994. Diversity in wild and cultivated species of Sesamum and its use. In: R.K. Arora & K.W. Riley (Eds), Sesame Biodiversity in Asia — Conservation, Evaluation and Improvement. pp 13-23, International Plant Genetic Resources Institute, Office for South Asia, New Delhi.Google Scholar
  27. Welsh, J. & M. McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acid Research 18: 7213-y7218.Google Scholar
  28. Williams, J.G.K., A.R. Kubelic, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research 18: 6531-6535.Google Scholar
  29. Yermamos, D.M., 1980. Sesame In: W.R. Fehr & H.H. Hadley (Eds), Hybridisation of Crop Plants. Am Soc Agron, CSSA, Madison, Wisconsin, USA.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • K. Venkataramana Bhat
    • 1
  • Prashant P. Babrekar
    • 1
  • Suman Lakhanpaul
    • 1
  1. 1.National Research Centre on DNA FingerprintingNational Bureau of Plant Genetic Resources, Pusa CampusNew Delhi-India

Personalised recommendations