Euphytica

, Volume 107, Issue 3, pp 185–192 | Cite as

Resistance to powdery mildew in a doubled haploid barley population and its association with marker loci

  • I. Falak
  • D.E. Falk
  • N.A. Tinker
  • D.E. Mather
Article

Abstract

The genetic basis of resistance to powdery mildew (Erysiphe graminis DC. f.sp. hordei Marchal) was analyzed using doubled haploid barley (Hordeum vulgare L.) lines from the cross Harrington/TR306. Based on infection types observed after inoculation with defined single-conidium isolates, the lines were classified into four groups. The observed phenotypic ratio fit a two-locus model. The two putative loci were mapped relative to molecular markers. One coincided with the previously mapped dMlg locus on chromosome 4. Based on the observed infection types, Harrington carries the Mlg resistance allele, and TR306 carries a second locus on chromosome 7 (5H); this was tentatively designated Ml(TR). It is the first reported race-specific powdery mildew resistance gene located on that chromosome. These two loci were also detected by simple interval mapping of disease severity data from naturally infected field plots. Composite interval mapping with the first two resistance loci as co-factors detected an additional locus on chromosome 6, with a minor effect on resistance. Finally, superimposing the race-specific classification onto the field data provided evidence for a minor-effect locus on chromosome 7 (5H). The Mlg locus had the largest effect, the Ml(TR) locus had an intermediate effect and the other two loci had very small effects. This study demonstrates the effectiveness of an integrated approach to identifying and mapping resistance loci using classification data from inoculated experiments and quantitative data from field experiments.

barley Erysiphe graminisDC. f.sp. hordei Marchal genetic mapping Hordeum vulgare L. partial resistance QTL specific resistance 

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References

  1. Backes, G., A. Graner, B. Foroughi-Wehr, G. Fishbeck, G. Wenzel & A. Jahoor, 1995. Localization of quantitative trait loci (QTL) for agronomicly important characters by the use of a RFLP map in barley (Hordeum vulgare L.). Theor. Appl. Genet. 90: 294–302.CrossRefGoogle Scholar
  2. Falak, I. & D.E. Falk, 1993. Doubled haploids as a tool for studying resistance to powdery mildew (Erysiphe graminis f.sp. hordei). Barley Newsletter 36: 208.Google Scholar
  3. Falak I., 1994. Analysis of a doubled haploid population for resistance to powdery mildew. M.Sc. Thesis. University of Guelph, Guelph, Ont. Canada. 168 pp.Google Scholar
  4. Haley, C.S. & S.A. Knott, 1992. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69: 315–324.PubMedGoogle Scholar
  5. Hayes, P.M., B.H. Liu, S.J. Knapp, F. Chen, B.L. Jones, T. Blake, J. Franckowiak, D. Rasmusson, M. Sorrells, S.E. Ullrich, D. Wesenberg & A. Kleinhofs, 1993. Quantitative trait locus effects and environmental interaction in a sample of North American barley germ plasm. Theor. Appl. Genet. 87: 392–401.CrossRefGoogle Scholar
  6. Heun, M., 1987. Genetics of quantitative resistance in barley against Erysiphe graminis f.sp. hordei. In: S. Yasuda & T. Konishi (Eds), Barley Genetics V, pp. 593–600. Okayama University Press, Japan.Google Scholar
  7. Heun, M., 1992. Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map. Genome 35: 1019–1025.Google Scholar
  8. Jørgensen, J.H., 1987. Three kinds of powdery mildew resistance in barley. In: S. Yasuda & T. Konishi (Eds.), Barley Genetics V, pp. 583–592. Okayama University Press, Japan.Google Scholar
  9. Jørgensen, J.H., 1994. Genetics of powdery mildew resistance in barley. CRC Critical Reviews in Plant Sciences. 13: 97–119.Google Scholar
  10. Kasha, K.J., A. Kleinhofs, A. Kilian, M. Saghai Maroof, G.J. Scoles, P.M. Hayes, F.Q. Chen, X. Xia, X.-Z. Li, R.M. Biyashev, D. Hoffman, L. Dahleen, T.K. Blake, B.G. Rossnagel, B.J. Steffenson, P.L. Thomas, D.E. Falk, A. Laroche, W. Kim, S.J. Molnar & M.E. Sorrells, 1995. The North American barley map on the cross HT and its comparison to the map on cross SM. In: K. Tsunewaki (Ed.), The Plant Genome and Plastome: Their Structure and Evolution, pp. 73–88. Kodansha Scientific Ltd., Tokyo.Google Scholar
  11. Kleinhofs, A., A. Kilian, M.A. Saghai Maroof, R.M. Biyashev, P. Hayes, F.Q. Chen, N. Lapitan, A. Fenwick, T.K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, D. Kudrna, J. Bollinger, S.J. Knapp, B. Liu, M. Sorrells, M. Heun, J.D. Franckowiak, D. Hoffmann, R. Skadsen & B.J. Steffenson, 1993. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor. Appl. Genet. 86: 705–712.CrossRefGoogle Scholar
  12. Knudsen, J.C.N., H.H. Dalsgaard & J.H. Jørgensen, 1987. Partial resistance to powdery mildew. In: S. Yasuda & T. Konishi (Eds.), Barley Genetics V, pp. 645–666. Okayama University Press, Japan.Google Scholar
  13. Kølster, P. & O. Stølen, 1987. Barley isolines with genes for resistance to Erysiphe graminis f.sp. hordei in the recurrent parent Siri. Plant Breeding 98: 79–82.CrossRefGoogle Scholar
  14. Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–178.PubMedCrossRefGoogle Scholar
  15. Mather, D.E., N.A. Tinker, D.E. LaBerge, M. Edney, B.L. Jones, B.G. Rossnagel, W.G. Legge, K.G. Briggs, R.B. Irvine, D.E. Falk & K.J. Kasha, 1997. Regions of the genome that affect grain and malt quality in a North American two-row barley cross. Crop Sci. 37: 544–554.CrossRefGoogle Scholar
  16. Moseman, J.G., 1972. Isogenic barley lines for reaction to Erysiphe graminis f.sp. hordei. Crop Sci. 12: 681–682.CrossRefGoogle Scholar
  17. Saghai Maroof, M.A., Q. Zhang & R.M. Biyashev, 1994. Molecular marker analysis of powdery mildew resistance in barley. Theor. Appl. Genet. 88: 733–740.CrossRefGoogle Scholar
  18. Spaner, D., L.P. Shugar, T.M. Choo, I. Falak, K.G. Briggs, W.G. Legge, D.E. Falk, S.E. Ullrich, N.A. Tinker, B.J. Steffenson & D.E. Mather, 1998. Mapping of disease resistance loci in barley on the basis of naturally occurring symptoms. Crop Science 38: 843–850.CrossRefGoogle Scholar
  19. Thomas, W.T.B., W. Powell, R. Waugh, K.J. Chalmers, U.M. Barua, P. Jack, V. Lea, B.P. Forster, J.S. Swanston, R.P. Ellis, P.R. Hanson & R.C.M. Lance, 1995. Detection of quantitative trait loci for agronomic, yield, grain and disease characters in spring barley (Hordeum vulgare L.). Theor. Appl. Genet. 91: 1037–1047.CrossRefGoogle Scholar
  20. Tinker, N.A. & D.E. Mather, 1995a. Methods for QTL analysis with progeny replicated in multiple environments. J. Quantitative Trait Loci 1:1 (http://probe.nalusda.gov:8000/otherdocs/jqtl/1995–01/jqtl15.html).Google Scholar
  21. Tinker, N.A. & D.E. Mather, 1995b. MQTL: software for simplified composite interval mapping of QTL in multiple environments. J. Quantitative Trait Loci 1: 2. (http://probe.nalusda.gov:8000/otherdocs/jqtl/995–02/jqtl16r2.html).Google Scholar
  22. Tinker, N.A., D.E. Mather, B.G. Rossnagel, K.J. Kasha, A. Kleinhofs, P.M. Hayes, D.E. Falk, T. Ferguson, L.P. Shugar, W.G. Legge, R.B. Irvine, T.M. Choo, K.G. Briggs, S.E. Ullrich, J.D. Franckowiak, T.K. Blake, R.J. Graf, S.M. Dofing, M.A. Saghai Maroof, G.J. Scoles, D. Hoffman, L.S. Dahleen, A. Kilian, F. Chen, R.M. Biyashev, D.A. Kudrna & B.J. Steffenson, 1996. Regions of the genome that affect agronomic performance in two-row barley. Crop Sci. 36: 1053–1062.CrossRefGoogle Scholar
  23. Wiberg, A., 1974. Genetical studies of spontaneous sources of resistance to powdery mildew in barley. Hereditas 77: 89–148.PubMedCrossRefGoogle Scholar
  24. Wolfe, M.S., U. Brandle, B. Koller, E. Limpert, J.M. McDermott, K. Muller & D. Schaffner, 1992. Barley mildew in Europe: population biology and host resistance. Euphytica 63: 125–139.CrossRefGoogle Scholar
  25. Zadoks, J.C., T.T. Chang & C.F. Konzak, 1974. A decimal code for the growth stages of cereals. Weed Res. 14: 415–421.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • I. Falak
    • 1
  • D.E. Falk
    • 1
  • N.A. Tinker
    • 2
  • D.E. Mather
    • 2
  1. 1.Crop Science Division of the Plant Agriculture DepartmentUniversity of GuelphGuelphCanada
  2. 2.Department of Plant ScienceMcGill UniversitySte-Anne-de-BellevueCanada

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