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The Histochemical Journal

, Volume 31, Issue 4, pp 241–243 | Cite as

High Efficiency Gene Transfer by Multiple Transfection Protocol

  • Manabu Yamamoto
  • Satoshi Okumura
  • Carsten Schwencke
  • Junichi Sadoshima
  • Yoshihiro Ishikawa
Article

Abstract

A high efficiency transfection protocol employing a common polycationic lipid is described. Using LipofectAMINE, a widely used transfection reagent, we transfected 293T cells with a plasmid harboring the β-galactosidase (β-gal) gene. The transfection efficiency was determined by direct staining for X-gal. The conventional transfection protocol achieved an efficiency of <40% while our protocol, which employs the repetition of transfection a few times, achieved an efficiency of approximately 80%. Thus, a dramatic increase in transfection efficiency can be obtained by simply repeating transfection with the use of a common polycationic lipid. This method will be useful in many molecular biological experiments.

Keywords

Lipid Gene Transfer 293T Cell Transfection Efficiency Transfection Reagent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Manabu Yamamoto
    • 1
  • Satoshi Okumura
    • 1
  • Carsten Schwencke
    • 1
  • Junichi Sadoshima
    • 1
  • Yoshihiro Ishikawa
    • 1
  1. 1.Cardiovascular & Pulmonary Research Institute, AlleghenyUniversity of the Health SciencesPittsburghUSA

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