Euphytica

, Volume 106, Issue 2, pp 187–195 | Cite as

Development of a PCR-based selection assay for root-knot nematode resistance ( Rmc1) by a comparative analysis of the Solanum bulbocastanum and S. tuberosum genome

  • Jeroen N.A.M. Rouppe van der Voort
  • Geert J.W. Janssen
  • Hein Overmars
  • Peter M. van Zandvoort
  • Arend van Norel
  • Olga E. Scholten
  • Richard Janssen
  • Jaap Bakker
Article

Abstract

A PCR-based assay has been developed for marker assisted selection of root-knot nematode resistance ( Rmc1) in potato. To this end, a comparative genome analysis was carried out between Solanum bulbocastanum and S. tuberosum to identify PCR-based chromosome 11 alleles linked to Rmc1. The use of co-migrating AFLP markers, obtained by using primer combinations previously applied for AFLP analysis of the S. tuberosum genome, failed to align the AFLP map of the S. bulbocastanum genome with the S. tuberosum map. Apparently, the S. bulbocastanum genome is genetically too distantly related to the S. tuberosum genome for this type of analysis. Cleaved amplified polymorphic sequence (CAPS) markers were more readily applied for a comparative analysis within the region of interest. Rmc1 could be localized within a 4 cM interval between markers CT182 and M39b. It is demonstrated that the resistance spectrum of Rmc1 includes not only Meloidogyne chitwoodi and the related species M. fallax but also a genetically distinct population of M. hapla. The cost-efficiency of the CAPS markers applied for Rmc1 renders this approach as an attractive alternative for screening large segregating populations of potato for root-knot nematode resistance.

AFLP CAPS comparative mapping marker assisted selection Meloidogyne ssp. 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Jeroen N.A.M. Rouppe van der Voort
    • 1
  • Geert J.W. Janssen
    • 2
  • Hein Overmars
    • 1
  • Peter M. van Zandvoort
    • 1
  • Arend van Norel
    • 2
  • Olga E. Scholten
    • 2
  • Richard Janssen
    • 2
  • Jaap Bakker
    • 1
  1. 1.Department of NematologyThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural UniversityWageningenThe Netherlands
  2. 2.DLO-Centre for Plant Breeding and Reproduction Research (CPRO- DLO)WageningenThe Netherlands

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