Molecular mapping of a new genic male-sterility gene causing chalky endosperm in rice ( Oryza sativa L.)
- 317 Downloads
A genic male-sterility gene newly induced by chemical mutagenesis, tentatively designated as ms-h(t), was located on the molecular map of rice and tested for its effect on chalky endosperm. Bulked segregant analysis was used to determine the chromosomal location of the ms-h(t) locus by screening four to five RFLP markers per chromosome. After confirming that the gene was located on chromosome 9, twenty-four RFLP markers from chromosome 9 were surveyed for polymorphism in the parents of the mapping population. Of these, eleven markers were mapped around the ms- h(t) locus. RG451 and RZ404 flanked the ms-h(t) gene, at 2.5cM and 3.3cM, respectively. Heterozygous F2 to F4 progenies were tested for co- segregation of male-sterility and chalky endosperm and it was revealed that ms-h(t) might have a pleiotropic effect on chalky endosperm. This mutant would be a good biological material to characterize the biochemical mechanism of male sterility and related pleiotropic effects. Further studies should be needed to know the usefulness of this mutant for hybrid seed production.
Unable to display preview. Download preview PDF.
- Galinat, W.C., 1975. Use of male sterile 1 gene to eliminate detasseling in production of hybrid seed of bicolor sweet corn. J Hered 66: 387–388.Google Scholar
- Hoshikawa, K., 1993. Rice grain texture and grading standard. In: T. Matsuo & K. Hoshilawa (Eds), 'Science of the Rice Plant, pp. 383–389. Vol.1. Morphology, Food and Agriculture Policy Research Center, Tokyo, Japan.Google Scholar
- Kaul, M.L.H., 1988. Male Sterility in Higher Plant. Springer-Verlag, pp. 15–96.Google Scholar
- Kinoshita, T., 1995. Report of committee on gene symbolization, nomenclature and linkage groups. Rice Genet Newsl 12: 9–153.Google Scholar
- Ko, T. & H. Yamagata, 1987. Gene analysis of male sterility induced in rice. Studies on the utility of artificial mutations in plant breeding. XVI. Jpn J Breed 37: 192–198.Google Scholar
- Ko, T. & H. Yamagata, 1989. Studies on the induction of malesterile strains in rice. 9. Linkage groups of a few male-sterility genes. Jpn J Breed 39 (Suppl. 1): 234–235.Google Scholar
- Koh, H.J. & M.H. Heu, 1996. Agronomic characteristics of a mutant for genic male sterility-chalky endosperm and its utilization on F1 hybrid breeding system in rice. Korean J Crop Sci 40(6): 684–696.Google Scholar
- Kosambi, D.D., 1944. The estimation of map distances from recombination values. Ann Eugenet 12: 172–175.Google Scholar
- Lu, X.G., Z.G.Zhang, K. Maruyama & S.S. Virmani, 1994. Current status of two-line method of hybrid rice breeding. In: S.S. Virmani (Ed), Hybrid Rice Technology — New Developments and Future Prospects, pp. 37–49. IRRI.Google Scholar
- Michelmore, R.W., I. Paran & K.V. Kesseli, 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832.PubMedCrossRefGoogle Scholar
- Trees, S.C. & J.N. Rutger, 1978. Inheritance of four genetic male steriles. J Hered 69: 270–272.Google Scholar