Euphytica

, Volume 105, Issue 3, pp 167–172 | Cite as

Production and testing of plants regenerated from protoplasts of photoperiod sensitive genic male sterile rice (Oryza sativa L.)

  • Qingzhong Xue
  • Kossi Edoh
  • Hong Li
  • Nengyi Zhang
  • Juqiang Yan
  • Susan McCouch
  • Elizabeth D. Earle
Article

Abstract

Plants were regenerated from protoplasts isolated from embryonic suspension cultures of N5047S, a photoperiod sensitive genic male sterile (PGMS) Japonica rice line. Flow cytometric analyses of nuclear DNA content identified some tetraploid regenerates whose agronomic traits could be distinguished from diploid regenerates. Pollen and female fertility of diploid protoplast-derived clones grown under different light and temperature conditions was compared. A promising PGMS protoplast clone, ZAU11S, was developed from these clones. Its male sterility was confirmed as a photoperiod × temperature interaction type.

rice Oryza sativa L. photoperiod sensitive genic male sterility (PGMS) protoplasts flow cytometry tetraploid 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Qingzhong Xue
    • 1
  • Kossi Edoh
    • 1
  • Hong Li
    • 1
  • Nengyi Zhang
    • 1
  • Juqiang Yan
    • 1
  • Susan McCouch
    • 2
  • Elizabeth D. Earle
    • 2
  1. 1.Department of AgronomyZheijiang Agricultural UniversityHangzhouChina
  2. 2.Department of Plant BreedingCornell UniversityIthacaU.S.A

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