Hydrobiologia

, Volume 359, Issue 1–3, pp 159–162

Extraction of DNA from anostracan cysts (Crustacea, Branchiopoda) for use in RAPD-PCR analyses

  • Jacob A. Moorad
  • Michael S. Mayer
  • Marie A. Simovich
Article

DOI: 10.1023/A:1003154618381

Cite this article as:
Moorad, J.A., Mayer, M.S. & Simovich, M.A. Hydrobiologia (1997) 359: 159. doi:10.1023/A:1003154618381

Abstract

Like many diapausing crustaceans, anostracans (Crustacea, Branchiopoda) produce encysted embryos capable of surviving the long periods of desiccation typical of their environments. These cysts are far more abundant and are easier to collect than adults and for some applications they are superior to adults as subjects for genetic analysis. Due to the lack of minimal tissue size requirements, PCR-based analysis is the best alternative for genetic study of cysts. We describe a method for the fast extraction of DNA from cysts, yielding template for RAPD-PCR analysis.

Crustacea Anostraca cysts egg bank RAPD PCR 

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Jacob A. Moorad
    • 1
  • Michael S. Mayer
    • 2
  • Marie A. Simovich
    • 2
  1. 1.Department of Marine and Environmental ScienceUniversity of San DiegoUSA
  2. 2.Department of BiologyUniversity of San DiegoUSA

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