, Volume 365, Issue 1–3, pp 281–285 | Cite as

Ecdysteroids in nemerteans: further characterization and identification

  • Robert K. Okazaki
  • Mark J. Snyder
  • Casey C. Grimm
  • Ernest S. Chang


Ecdysteroids are a class of steroidal hormones thatare important in molting and reproduction inarthropods. These hormones have been recentlydetected in non-arthropodan groups, such assoft-bodied worms. To continue our efforts todetermine the presence of ecdysteroids in nemerteans,this study further documents the identification of20-hydroxyecdysone (20E), in Paranemertesperegrina, by gas chromatography/mass spectrometry(GC/MS). After C18 Sep-Pak extraction,radioimmunoassay of the 40% and 80% methanolicfractions from an extract of 1000 worms indicated 98and 156 ng of ecdysteroids, respectively. Ecdysteroids of these two samples, as well as the 20Eand ecdysone standards, were N-trimethylsilyimidazole(TMSI)-derivatized before GC/MS analysis. Themethanolic samples contained a large number ofcompounds but only small, insignificant peaks in thearea where ecdysteroid standards eluted. However, thereconstruction ion current (RIC) chromatograms for m/z561 indicated the presence of 20E at the correctretention time of 12.48 min in the 40% methanolicfraction. Reanalysis of the samples under selectedion monitoring mode demonstrated the presence of 20Ein both the 40% and 80% fractions. These resultsdemonstrate conclusively the presence of an activeecdysteroid in the phylum Nemertea.

nemertean Paranemertesperegrina ecdysteroid GC-MS RIA 


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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Robert K. Okazaki
    • 1
  • Mark J. Snyder
    • 2
  • Casey C. Grimm
    • 3
  • Ernest S. Chang
    • 2
  1. 1.Department of Biological SciencesSoutheastern Louisiana UniversityHammondU.S.A
  2. 2.Bodega Marine LaboratoryUniversity of CaliforniaBodega BayU.S.A
  3. 3.Southern Regional Research Center, Agricultural Research ServiceUnited States Department of AgricultureNew OrleansU.S.A

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