Biologia Plantarum

, Volume 42, Issue 4, pp 481–497 | Cite as

Acclimatization of Micropropagated Plants to Ex Vitro Conditions

  • J. Pospóšilová
  • I. Tichá
  • P. Kadleček
  • D. Haisel
  • Š. Plzáková


The special conditions during in vitro culture result in the formation of plantlets of abnormal morphology, anatomy and physiology. After ex vitro transfer, these plantlets might easily be impaired by sudden changes in environmental conditions, and so need a period of acclimatization to correct the abnormalities. This review is focused upon contemporary information on the changes in leaf structure, water relations and photosynthesis during acclimatization of plantlets to ex vitro conditions. It also describes some ways of improving plant survival and for the speeding up of acclimatization.

antitranspirants chlorophyll content chlorophyll fluorescence cuticle epidermis hardening in vitro and ex vitro growth leaf mesophyll net photosynthetic rate photoautotrophy photosynthetic rate photoautotrophy photoinhibition photomixotrophy stomatal conductance stomatal density transpiration rate water potential 


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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • J. Pospóšilová
    • 1
  • I. Tichá
    • 2
  • P. Kadleček
    • 2
  • D. Haisel
    • 1
  • Š. Plzáková
    • 2
  1. 1.Institute of Experimental Botany, Academy of Sciences of the Czech RepublicPraha 6Czech Republic
  2. 2.Faculty of Science, Department of Plant PhysiologyCharles UniversityPraha 2Czech Republic

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